pjp5`2002.vp:CorelVentura 7.0

Copyright © 2002 by Institute of Pharmacology
Polish Academy of Sciences
Polish Journal of Pharmacology
Pol. J. Pharmacol., 2002, 54, 495–500
ISSN 1230-6002
DISTRIBUTION OF ALLELIC VARIANTS OF FUNCTIONAL
C3435T POLYMORPHISM OF DRUG TRANSPORTER
MDR1 GENE IN A SAMPLE OF POLISH POPULATION
Krzysztof Jamroziak1,#, Ewa Balcerczak2, Wojciech M³ynarski3,
Marek Mirowski2, Tadeusz Robak1
Department of Haematology, Medical University of £ódŸ, Cio³kowskiego 2, PL 93-510 £ódŸ, Poland,
Department of Pharmaceutical Biochemistry, Molecular Biology Laboratory, Muszyñskiego 1, PL 90-151 £ódŸ, Poland,
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Clinic of Pediatrics, Institute of Pediatrics at Medical University of £ódŸ, Sporna 36/50, PL 91-738 £ódŸ, Poland
Distribution of allelic variants of functional C3435T polymorphism of
drug transporter MDR1 gene in a sample of Polish population. K. JAMROZIAK, E. BALCERCZAK, W. M£YNARSKI, M. MIROWSKI, T. ROBAK.
Pol. J. Pharmacol., 2002, 54, 495–500.
P-glycoprotein (P-gp), the protein product of MDR1 gene, is an important factor regulating the bioavailability of many therapeutics. Recently, the
C3435T polymorphism of MDR1 was correlated to altered expression and
function of P-gp in normal tissues. In this study, polymerase chain reaction –
restriction fragment length polymorphism (PCR-RFLP) assay was applied to
assess C3435T MDR1 polymorphism in 122 healthy individuals of Slavic
origin from the population of central Poland (£ódŸ and surrounding areas).
The detected genotype variant frequencies were as follows: CC in 42%, CT
in 41%, and TT in 17% of the tested subjects (C-allele frequency was 0.62).
The frequency of the C-allele is similar to Japanese population and significantly higher than in Caucasians from Western Europe. The results of this
study give basis for large-scale C3435T MDR1 genotype-phenotype correlation investigations in Polish population that may be useful to individualize
therapy of cancer, HIV-1 infection and some other diseases.
Key words: MDR1, P-glycoprotein, C3435T polymorphism, pharmacogenetics
correspondence; e-mail: [email protected]