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Vol. 52 No. 4/2005 QUARTERLY PL ISSN 0001-527X 31 11 13 on-line at: www.actabp.pl Contents In Memoriam Professor Maciej Wiewiórowski Reviews Protein modeling with reduced representation: statistical potentials and protein folding mechanism Dariusz Ekonomiuk, Marcin Kielbasinski and Andrzej Kolinski 741 Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters Marzena Podgorska, Katarzyna Kocbuch and Tadeusz Pawelczyk 749 Short-term regulation of the mammalian pyruvate dehydrogenase complex Sławomir Strumiło 759 Regular papers Kinetic analysis of the transient phase and steady state of open multicyclic enzyme cascades Ramón Varón, Bent H. Havsteen, Edelmira Valero, Milagros Molina-Alarcón, Francisco García-Cánovas and Manuela García-Moreno 765 Thermostable Pyrococcus woesei β-�-galactosidase — high level expression, purification and biochemical properties Marta Wanarska, Józef Kur, Radosław Pladzyk and Marianna Turkiewicz 781 Isolation and characterization of pigeon breast muscle cytosolic 5´-nucleotidase-I (cN-I) Kinga Tkacz-Stachowska, Katarzyna Lechward and Andrzej C. Skladanowski 789 The role of benzoate secreted by Desulfotomaculum acetoxidans DSM 771 in sulfate uptake Lucyna Pawłowska-Ćwięk and Ryszard Pado 797 Time-dependent effect of leptin on renal Na+,K+-ATPase activity Andrzej Marciniak, Anna Jamroz-Wiśniewska, Ewelina Borkowska and Jerzy Bełtowski 803 The role of Na+/H+ exchanger in serotonin secretion from porcine blood platelets Marian Tomasiak, Michal Ciborowski and Halina Stelmach 811 The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome Marcin T. Schmidt, Agnieszka K. Olejnik and Anna Goździcka-Józefiak 823 Two types of non-homologous RNA recombination in brome mosaic virus Magdalena Alejska, Nelli Malinowska, Anna Urbanowicz, Marek Figlerowicz 833 Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves Mireia Farriol and Xavi Orta 845 Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA Janusz Szemraj, Khalid N.I. Al-Nedawi, Ewa Chabielska, Wlodzimierz Buczko and Zofia Pawlowska 849 Ribosomal proteins L34 and S29 of amphioxus Branchiostoma belcheri tsingtauense: cDNAs cloning and gene copy number Lina Liu, Shicui Zhang, Zhenhui Liu, Hongyan Li, Mei Liu, Yongjun Wang and Lifang Ma 857 Expression of recombinant forms of human 21.5 kDa myelin basic protein and proteolipid protein in CHO cells Ewa Jaśkiewicz, Anna Jedynak and Ewa Zioło 863 Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis-diamminedichloroplatinum Monika Pietrowska and Piotr Widłak 867 Recombinant angioarrestin secreted from mouse melanoma cells inhibits growth of primary tumours Andrzej Smagur, Jarosław Szary and Stanisław Szala 875 Expression and hypoxia-responsiveness of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 in mammary gland malignant cell lines Oleksandr H. Minchenko, Iryna L. Opentanova, Tsutomu Ogura, Dmytro O. Minchenko, Sergiy V. Komisarenko, Jaime Caro and Hiroyasu Esumi 881 The importance of abrogation of G2-phase arrest in combined effect of TRAIL and ionizing radiation Martina Řezáčová, Jiřina Vávrová, Doris Vokurková, Aleš Tichý, Jiří Knížek and Jan Psutka 889 The effect of Topotecan on oxidative stress in MCF-7 human breast cancer cell line Mujgan Timur, S. Halide Akbas and Tomris Ozben 897 Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher Yasuhiro Kambayashi, Susumu Takekoshi, Minoru Nakano, Masafumi Shibamori, Yoshiaki Hitomi and Keiki Ogino 903 Effect of aging and oxidative/genotoxic stress on poly(ADP-ribose) polymerase-1 activity in rat brain Robert P. Strosznajder, Henryk Jesko and Agata Adamczyk 909 New spectrophotometric methods for the determination of nifedipine in pharmaceutical formulations Nafisur Rahman and Syed Najmul Hejaz Azmi 915 Communications Methylxanthines (caffeine, pentoxifylline and theophylline) decrease the mutagenic effect of daunomycin, doxorubicin and mitoxantrone Jacek Piosik, Anna Gwizdek-Wiśniewska, Katarzyna Ulanowska, Jakub Ochociński, Agata Czyż and Grzegorz Węgrzyn 923 Modulation of ERK1/2 activity is crucial for sphingosine-induced death of glioma C6 cells Patryk Krzemiński 927 Does in vitro replicative senescence of human CD8+ cells reflect the phenotypic changes observed during in vivo ageing? Agnieszka Brzezinska 931 Role of NAD(P)H : quinone oxidoreductase (NQO1) in apoptosis induction by aziridinylbenzoquinones RH1 and MeDZQ Aušra Nemeikaitė-Čėnienė, Aldona Dringelienė, Jonas Šarlauskas and Narimantas Čėnas 937 Isolation and expression pa�ern of RGS21 gene, a novel RGS member Xin Li, Lei Chen, Chaoneng Ji, Bing Liu, Jiefeng Gu, Jian Xu , Xianqiong Zou, Shaohua Gu and Yumin Mao 943 Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae Katarzyna Domańska, Rafał Zieliński, Konrad Kubiński, Ewa Sajnaga, Maciej Masłyk, Maria Bretner and Ryszard Szyszka 947 The effect of new non-cross resistant antitumour agents on the energy state of human erythrocytes Robert Nowak, Irena Baranowska-Bosiacka, Barbara Stefańska, Bogusław Machaliński, Alina J. Hłyńczak and Jolanta Tarasiuk 953 Index of Authors Adamczyk AA Akbas SH Alejska M Al-Nedawi KNI Azmi SNH Baranowska-Bosiacka I Bełtowski J Borkowska E Bretner M Brzezinska A Buczko W Caro J Čėnas N Chabielska E Chen L Ciborowski M Czyż A Domańska K Dringelienė A Ekonomiuk D Esumi H Figlerowicz M Farriol M García-Cánovas F García-Moreno M Goździcka-Józefiak A Gu J Gu S Gwizdek-Wiśniewska A Havsteen BH Hitomi Y Hłyńczak AJ Jamroz-Wiśniewska A Jaśkiewicz E Jedynak A Jesko H Ji Ch Kambayashi Y Kielbasinski M Knížek J 909 897 833 849 915 953 803 803 947 931 849 881 937 849 943 811 923 947 937 741 881 833 845 765 765 823 943 943 923 765 903 953 803 863 863 909 943 903 741 889 Kocbuch K Kolinski A Komisarenko SV Krzemiński P Kubiński K Kur J Lechward K Li H Li X Liu B Liu L Liu M Liu Z Ma L Machaliński B Malinowska N Mao Y Marciniak A Masłyk M Minchenko DO Minchenko OH Molina-Alarcón M Nakano M Nemeikaitė-Čėnienė A Nowak R Ochociński J Ogino K Ogura T Olejnik AK Opentanova IL Orta X Ozben T Pado R Pawelczyk T Pawlowska Z Pawłowska-Ćwięk L Pietrowska M Piosik J Pladzyk R Podgorska M 749 741 881 927 947 781 789 857 943 943 857 857 857 857 953 833 943 803 947 881 881 765 903 937 953 923 903 881 823 881 845 897 797 749 849 797 867 923 781 749 Psutka J Rahman N Řezáčová M Sajnaga E Šarlauskas J Shibamori M Skladanowski AC Smagur A Stefańska B Stelmach H Schmidt MT Strosznajder RP Strumiło S Szala S Szary J Szemraj J Szyszka R Takekoshi S Tarasiuk J Tichý A Timur M Tomasiak M Turkiewicz M Tkacz-Stachowska K Ulanowska K Urbanowicz A Valero E Varón R Vávrová J Vokurková D Wanarska M Wang Y Węgrzyn G Widłak P Xu J Zhang S Zieliński R Zioło E Zou X 889 915 889 947 937 903 789 875 953 811 823 909 759 875 875 849 947 903 953 889 897 811 781 789 923 833 765 765 889 889 781 857 923 867 943 857 947 863 943