r markeraiSSthe detection of the wheat leaf rust and powdery mitó

r markeraiSSthe detection of the wheat leaf rust and powdery mitó'.|?w^ie^tańcę(geńe^iń MAS
K SANDRA P IE T R U S IŃ S K A . JE R Z Y HENRYK CZEMBOR
Department of Plant Breeding and Genetics
ion Institute - National Research Institute at Radzików, 0 5 -8 7 0 Błonie, Poland
e -m a il: [email protected]
INTRODUCTION
Leaf rust, caused b y Puccinia recondita f.s p . tritid n a , and powdery mildew, caused b y B hm eria gram inis f.s p . tritic i. are the most prevalent of all of the
world wheat diseases in many regions o f the world including Poland. These two diseases can cause serious epidemics in Poland. Highly effective strategy in the
present plant breeding programs is an introduction o f several resistance genes into one genotype. Pyramiding resistance genes f o r leaf rust and powdery mildew
combined with the M A 5 is very effective process to obtain new cultivars resistant to these diseases. Moreover, in breeding programs there a rc several
examples o f the successfully introduction resistance genes into a single cultivar and molecular markers are commonly used in this process. Th e re a re several
reports on introduction L r and Pm genes into wheat: L r l0 -L r l3 * L r l4 b * L r 3 O » L r 3 4 -Y r l8 into Opota 89 , Lr3 »Lrl7 »Lr2 3 * u n kn o w n L r, Pm4a*Pm21. Pm2«Pm21.
Pm2*Pm4a in th e Chinese Yang 158. Pm2*Pm5. Pm2*Pm6. Pm3d*Pm4b. Pm3d*Pm4b*Pm. Pml-Pm2*Pm9*Pm4b in the Polish wheat cultivars [1 ;2 ].
GOAL
The
aim o f
th e
presented
research
is gene
pyramiding
of
leaf
rust
and
powdery mildew
resistance
genes,
like (L r4 1 «P m 2 1 * lr4 7 ).
(Lr41 ♦Pm21 *Pm37),
(Lr41*Lr47«Pm 21*Pm 37). (Lr41 •Lr55*Pm37) and (Lr4 1 -Lr5 5 * P m 2 1 -P m 3 7 ) in a one genotype. T h e second objective o f oue study is to c la rify the location L r5 9 gene in wheat
genome and mapping closely linked molecular markers suitable fo r markers assisted selection (M A S ).
PARENTAL PLANT
1.
Recurrent Parents (RP) • 4 varietés of winter wheat: Nadobna. Bogatka, Muszelka. R AH979.
2.
Donor lines:
•
line KS90W GRC10 • Lr39 (* L r4 1 ) gene, os the sources of resistance to leaf rust derived from T. tovschii.
line UC996610010 * Lr47 gene, as the sources of resistance to leaf rust derived from T. spetfoides.
•
line KS04W SRC49 = Lr5 3 gene, as the sources o f resistance to leaf rust derived from B ym us fraehyeaalis.
•
line Yangmai 9 - Pm21 gene, as the sources of resistance to powdery mildew derived from Oasypyrum vittosum.
•
line N C 9 9 8 5 TA 1 1 • Pm37 gene, as the sources o f resistance to powdery mildew derived from T. timophccvii.
MOLECULAR ANALYSIS * FOREGROUND SELECTION
1.
Screening Lr3 9 gene: 6dm39, B a rc l2 4 . 6wm210,
6wm261. Gwm296.
2.
Screening Lr47 gene: (PCAPSR*PS10L*PS10L2).Gwm60.
3.
Screening Lr9 5 gene: D A r T ,
4.
Screening Pm21 gene: NAU/xiboo.
9.
Screening Pm37 gene: 6wm332, W m c790. STSB E4 06627
» ) A«|
I
(A . U 4 t )
1
I
t a l l ) m AA*
(A ■ IM I)
I
Lr41 gene
•J.t~.
f
r
•
•
:
i
S
;k
•cs<
(((A - L M 1) » (A m ► « » )) * A A ]
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1
Pm21 gene
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AA
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T
I
RESULTS
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pe/i)H |i«*ll4M *)ii M )
W t
■ AA)
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Owi •§
\
Up to now, the following populations were obtained:
{((Nadobna x L r4 1 ) x (Nadobna x Pm21) x Nadobna) x Nadobna) x Lr47 x Nadobna x Nadobna
2.
(((Nadobna x L r4 1 ) x (Nadobna x Pm21) x Nadobno) x Nadobna) x Pm37 x Nodobna x Nadobna
3.
(((Bogatka x L r4 1 ) x (Bogatka x Pm21) x Bogatka) x R AH979) x Lr4 7 x Nodobna x Nodobna
4.
(((Bogatka x L r4 1 ) x (Bogatka x P m 2ł) x Bogatka) x R AH979) x Lr4 7 x Bogatka x Bogatka
9.
(((Bogatka x L r4 1 ) x (Bogatka x Pm21) x Bogatka) x R AH979) x Pm37 x Nodobna x Nadobna
6.
(((Bogatka x L r4 1 ) x (Bogatka x Pm21) x Lexus) x M eteor) x Pm37 x Pm37 x Bogatka
7.
8.
9.
(((Nadobna x Lr4 1 ) x (Nadobna x Pm21) x Nadobno) x Nadobna) x Lr9 9 x Nadobna x Nadobna
|
*
k
I
4
T
1
1
Cxni •. K M I i ł a n . b « ) • M • M | ■
O
w
k
I
f . ym nww A
f- ętmmiim
t
i
T
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presented study are. the newly produced homozygous wheat lines which can be used as the source o f
effective resistance fo r the leaf rust and powdery mildew in breeding programs.
M •M ]
. A /ł . * /» )
i
■ ***" **i
H
■ AA ■ AA)
l
1
S
S
i
M f -p A a M
| (j4 lM M r 4 M i«)iiA / ( * AA)
■ AA ■ AA)
1
the foreground selection results can be sometimes problematic to interpret. T h e practical outcome o f
I
H
4
A / ł j . [ ( l s * |> M < l
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1
| (L M I> M l4 f « iła V )> A / ł * A A )
(I H I M K iiM M f ) * AA » AA]
summary, pyramiding of several resistance genes using m arker assisted proved th a t this
T h e resistance tests using differential set o f host and pathogen are essential in M A S , especially when
A/% .
P r4 l< # M tl* U 4 V ) .
SUMMARY
completely replace the traditional methods, especially f o r verification of the results in pathology tests.
A/t a A A )
W f -P I M W
X/* • AA)
(((Bogatka x L r4 1 ) x (Bogatka x Pm21) x Lexus) x M eteor) x L r59 x Bogatka x Bogatka
strategy is more efficient and speed up recurrent genome recovery. However, this approach can not
• AA)
AA ■
M
C
•Vi
(((Bogatka x L r4 1 ) x (Bogatka x Pm21) x R AH 979) x R AH 979) x L r4 7 x Bogatko x Bogatka
In
|K # « ^ l « n n < M T ) > w
•AA) > «•
(■Ci’ « * « * « ' " * ! •>»9> •
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* »l
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ł
MU
M
■a
k
4 a a
1
« « A
• A A ]> ■
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>MMOarmt <■«*-»»>•(»)
f S
rw itn w *4 S t M t n m : KT
T«w
REFERENCES
1 PietrunAsko A .. Czembor J . H .. Czembor P. Cz. 2011. P y ro »i* if of
two resistance genes for leaf rust and powdery mildew resistance in
common wheat. Cereal Research Comm. 39(4) 577-586
2 PietrunAsko A ., Czembor J . H ., Czembor P Cz. 2013. Lr39 • ft»21
a new effective combination of resistance genes for leof ru it and
powdery mildew. Czech Journal of Genetics ord Plont Breeding (in print)
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