Rozd 5.indd - Annals of Warsaw University of Life Sciences
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Rozd 5.indd - Annals of Warsaw University of Life Sciences
Annals of Warsaw University of Life Sciences – SGGW Animal Science No 54 (2), 2015: 153–160 (Ann. Warsaw Univ. of Life Sci. – SGGW, Anim. Sci. 54 (2), 2015) Effect of the backfat thickness at point P2 during insemination on the selected parameters of colostrum and milk of the sows ANNA REKIEL1, JUSTYNA WIĘCEK1, BEATA KUCZYŃSKA1, JUSTYNA BARTOSIK1, AGNIESZKA WARDA2, KATARZYNA FURMAN1 Department of Animal Breeding and Production, Warsaw University of Life Sciences – SGGW Polish Pig Breeders and Producers Association “POLSUS” 1 2 Abstract: Effect of the backfat thickness at point P2 during insemination on the selected parameters of colostrum and milk of the sows. The studies included 32 females F1 (PL × PLW), inseminated with the semen from Duroc boars. The females were assigned to two groups: L – “lean” sows: P2 ≤18 mm at mating (n = 16 heads), and F – “fat” sows: P2 >18 mm (n = 16 heads). The observations were carried on from the day of effective mating/insemination throughout the period of gestation and nutrition until weaning (35th day of lactation). Fatness of the sows (measurements of backfat thickness at points P2 and P4) and their muscling (height of M. longissimus dorsi at point P4M) at mating and in high pregnancy (104th ±1–2 day of gestation) were controlled. The samples of colostrum and milk were collected from all sows (64 samples in total). Basic chemical compositions, somatic cell count – SCC, and active acidity – pH, were determined. Thickness of backfat at point P2 did not have any influence on chemical composition of colostrum and milk, SCC and pH; in spite of it. The control of the sows’ condition is justified as there was indicated a significant difference (P ≤0.05) in the content of lactose (21st day) in favour of group F vs group L (0.56 percentage points). The mentioned result indicates the need of continuing the studies because lactose is a valuable, easily assimilated component of mother feed for the newborns. Key words: sows, backfat P2, quality of colostrum and milk INTRODUCTION Lactation – a part of lactation cycle, being understood as process of synthesis and production and secretion of milk by mammary glands of the females is the important factor of the mammals’ evolution (Rekiel 2003). The composition of milk was described in the XIX century (Rekiel 2006). Since the mentioned period, many publications dedicated to the composition and nutritive value of milk and to the factors affecting the level of milk production by the sows have appeared in scientific literature (Migdał et al. 2000, 2003, Rekiel 2003, 2006, Beyer et al. 2007, Beyga and Rekiel 2009, Farmer and Quesnel 2009, Rekiel et al. 2011, King’ori 2012, Szyndler-Nędza et al. 2013). Fat reserves (backfat thickness at P2 and P4) constitute the background for evaluation of the sows’ condition in five-score BCS scale (Body Condition Scoring), being a tool which enables the correct management of the herd (Rekiel and Beyga 2008, Bowker 2013). The quality and quantity of the produced colostrum and milk are dependent, i.a., on the condition, determined by protein 154 A. Rekiel et al. and fat reserves in the body (Beyga and Rekiel 2009, King’ori 2012). Klavier et al. (1981) found that the sows, which were heavier in parturition day as compared to the lighter ones, produced by 19% more milk during the first 12 days of lactation; the relationship of productivity and body weight was confirmed by King and Eason (1998). Beyga and Rekiel (2009) and Rekiel et al. (2011) demonstrated that the milk of the sows being in a good condition contained more fat and inconsiderably more calcium and phosphorus what progressively affected the rate of growth of the progeny. In the studies, there was also stated that the body weight and fatness and the dynamics of changes in fat reserves affected the pregnancy retention and reproduction performance (Walkiewicz et al. 1994, Koczanowski et al. 2000, Beyga and Rekiel 2009). Walkiewicz et al. (1994) found that in the case of the gilts with thicker backfat at mating (>15 mm) as compared to those ones with lower fat reserves (<15 mm), the level of fat in the milk was higher (on the 11th day of lactation by 1%). The domination was maintained during throughout the whole lactation, revealing, however, a decreasing tendency (on 21st and 42th day, the mentioned difference was equal to 0.4 and 0.1%, respectively). Similar differences and changes were recorded for dry solids content. The content of ash, protein and lactose was little differentiated. In the case of more fattened gilts, as compared to more lean ones, the level of unsaturated fatty acids C16:1 and C18:2 was higher. The aim of the studies was to determine the effect of fat reserves (thickness of backfat at P2 at mating/insemination) on the selected parameters of colostrum and milk of the sows of hybrids F1 (Polish Landrace × Polish Large White), i.e. basic chemical composition, active acidity (pH) and somatic cell count (SCC). MATERIAL AND METHODS The studies included 32 sows F1 (PL × × PLW), inseminated with the semen from Duroc boars. The females were assigned to two groups: L – “lean” sows: P2 ≤18 mm at mating (n = 16 heads), and F – “fat” sows: P2 >18 mm (n = 16 heads). The measurements of the backfat thickness and M. longissimus dorsi (MLD) in the sows were conducted at mating and in high pregnancy (104th ±1–2 day of gestation), using Pig-log 105 equipment according to method for evaluation of life performance of breeding animals (Eckert and Adamczyk 1993, Regulation of the Minister of Agriculture and Food Economy, Dz.U. 1999 nr 47, poz. 470, Regulation of the Minister of Agriculture and Rural Development, Dz.U. 2002 nr 169, poz. 1389). The observations were carried on from the day of effective mating/insemination throughout the period of gestation and nutrition until weaning (35th day of lactation). The intake of feed by the sows and fatness (at points P2 and P4) and musculature (height of MLD at P4M) were controlled. The samples of colostrum and milk were collected from the sows and evaluated (basic chemical composition, somatic cell count – SCC, Effect of the backfat thickness at point P2 during... and active acidity – pH (64 samples in total). During the experiment, the sows were fed individually with the full-ration mixture “pregnant’ sow (LP – sow in gestation) and “suckled” sow (LK – sow in lactation) – Table 1, in accordance with the Polish nutrient requirements for pigs (1993). The mentioned mixtures were prepared from maize and wheat meal, wheat bran and protein concentrate. The sows were kept in grouped pen from fourth week after insemination but the feed was administrated individually in feeding station, controlled by computer. The additional feeding of piglets with the mixture prestarter 5864 MPU 4% (ad libitum) was commenced at the end of the first week of life; after 3–4 weeks of rearing the mixture starter was introduced (Table 1). TABLE 1. Basic composition of the mixtures, employed in feeding of sows and piglets Mixtures Mixtures Basic comfor sows for piglets position Pre(%) LP LK Starter starter Dry matter 88.84 90.03 90.89 90.30 Crude ash 4.16 5.44 4.40 4.07 Crude 12.41 16.46 16.95 15.59 protein Crude fat 1.87 1.78 2.98 1.22 Crude fibre 5.21 2.77 3.10 2.93 155 geon and the piglets were subjected to standard care treatment. The basic analyses of the mixtures were performed according to AOAC procedures (2006). Colostrum was sampled from the sows in the day of parturition and the milk samples were collected on the 21st day of lactation, in the quantity of 50 ml/head, to the containers with preservative (Mlekostat CC). Before collecting the samples, the sows obtained oxytocin in injections (1 ml/100 kg body weight). For determination of somatic cell count (SCC) in colostrum and milk, Somacount 150 instrument by Bentley company, was employed. For determination of dry matter, protein, fat and lactose content by spectrophotometric method in infrared, the apparatus Milkoscan FT 120 by Foss Electric was used. For determination of active acidity (pH) the pH-meter pH 211 was employed. The results were statistically developed with utilization of package SPSS Statistics 21. The normality of distribution was checked by Shapiro-Wilk test. Somatic cell count was subjected to logarithmic transformation. The differences between the groups were checked by t-Student test (traits with a normal distribution) or U Mann-Whitney test (the remaining traits). RESULTS AND DISCUSSION The sows were kept for three weeks in individual pens and then, in groups. Ten days before farrowing, they were transported to three-part parturition pens in which they stayed until weaning of piglets at the age of five weeks. The animals were supervised by veterinary sur- The reserves of lipids (measurements of backfat thickness in points P2 and P4) and of protein (height of M. longissimus dorsi at point P4M) and evaluated life performance of meatiness are given in Table 2. The opinions on the thickness of backfat in gilts at the first mating are different. 156 A. Rekiel et al. TABLE 2. Thickness of backfat at points P2 and P4 and of M. longissimus dorsi at P4M and the assessed meatiness Group of sows Significance lean fat between the Traits groups SEM SEM x x Mating ** P2 15.05 0.484 23.48 0.618 ** P4 mm 18.30 0.649 25.23 0.911 P 4M 54.57 2.547 53.06 1.891 ns ** Meatiness % 53.71 0.812 46.86 0.718 104th day of gestation P2 21.20 1.085 23.29 0.873 ns ** P4 mm 20.75 0.992 25.74 0.820 P 4M 62.00 2.317 59.81 1.305 ns * Meatiness % 51.71 1.199 48.33 0.811 *Significant at P ≤0.05, **significant at P ≤0.001, ns – differences statistically insignificant. Dupas and Briend (1997) and Whittemore et al. (1995), after Grzyb et al. (2007), stated that the thickness of backfat in point P2 should be equal to 15 mm; according to Tummaruk et al. (2007), after Grzyb et al. (2007), for the gilts of hybrids, it should amount to 13–15 mm; and for the gilts of crossbreds of national breeds (PL × × PLW) – 19–20 mm (Rekiel et al. 2000). Besides it, Rekiel (2002) stated that at commencing the reproduction performance, the mean thickness of back fat should not be less than 16 mm. From the own studies, it is followed that the sows from group L had the mean thickness of backfat at point P2 somewhat lower (by 0.98 mm) than the recommended one (Table 2). The meatiness of the sows from group L vs group F differed significantly (P ≤0.001). Łyczyński et al. (2000), after Grzyb et al. (2007), informs that the improvement of meatiness causes decline of reproduction effectiveness. Rekiel and Więcek (2002a) revealed, however, that the gilts with a lower meatiness as primiparas delivered and reared more numerous litters. The results of the studies of Beyga and Rekiel (2009) and Szyndler-Nędza et al. (2013) confirmed the relationship of the sow’s condition and the reproduction traits and milk performance of the females and rearing of their progeny. In the own studies, differences in fatness and muscling were maintained in the sow groups L and F (comparative evaluation of the results of the studies from first and 104th day of gestation) what confirms the stability of lipid and protein reserves in their bodies. In case of the differentiation of the sows’ condition in groups F and L, any differences in basic composition and in somatic cell count (SCC and log SCC) and colostrum, as being statistically confirmed, were not found. All evaluated milk parameters in F and L groups of the sows were similar, except for the level of lactose. The content of lactic sugar was significantly (P ≤0.05) higher in milk of Effect of the backfat thickness at point P2 during... the sows from group F as compared to group L (difference equal to 0.56 percentage point – p.p.). The mentioned result is different than those ones obtained by Walkiewicz et al. (1994) and Revell et al. (1998) who recorded lower content of lactose in the milk of more fattened sows. The milk contained more lactose than colostrum in the group of the lean sows (by 1.72 p.p.) and the group of fat sows by 2.60 p.p. (Migdał et al. 2000, 2003). Paściak et al. (2003), Renaudaeu et al. (2003) inform that the level of lactose remained relatively stable and that one of dry matter, fat and protein varied. The results of the studies of Beyga and Rekiel (2009) indicate that the level of lactose in colostrum and milk of the sows which differed in fat reserves in high pregnancy stage (before parturition) was similar. As it was given by the cited authors, the level of lactose in colostrum was lower than in the milk and it was equal to about 3.5%; the results of the own studies are comparable with the cited literature. The level of lactose may become little increased during lactation. It is an important fact because it is the component of mother feed, being very well digested and assimilated by the piglets; it was revealed in the studies (Beyga and Rekiel 2009, King’ori 2012, Szyndler-Nędza et al. 2013) that the quality of colostrum and milk affected the results of the progeny rearing. Migdał et al. (2000, 2003), Rekiel (2003, 2006) and Beyga and Rekiel (2009) informed that the milk of the sows was characterized by a high content of 157 fat (about 7%). Fat of colostrum (considerably lower quantity than in milk) is valuable due to the higher participation of unsaturated fatty acids – UFA). In the own studies, any significant differences between the sow groups F and L in respect of fat content in colostrum as well as in milk were not found; it is the result being different than that one obtained by Beyga and Rekiel (2009). As compared to milk, the colostrum samples, collected from the sows, contained less fat, irrespectively of the group. The mentioned result was consistent with the literature data (Migdał et al. 2000, 2003, Beyga and Rekiel 2009). In the case of healthy sows, pH of colostrum is equal to 5.7–5.8; acidity of milk increases during the successive days after completion of parturition and reaches the level of 6.6 (Rekiel 2003). In the both examined groups of the sows, the acidity was correct (Table 3). Values for SCC and log SCC in colostrum and milk, as obtained in the own studies are comparable with the results of the studies of other authors (Rekiel and Więcek 2002b, Rekiel 2003, Beyga and Rekiel 2009). Values of SCC of the healthy sows should not exceed 0.5·106/ml. The result below 2.0·106/ml is recognized as correct although indicating the possibility of incidence of subclinical inflammatory state. Values of SCC on the level of 5.0·106/ml, being supplemented by the results of other diagnostic tests, constitutes the background for the diagnosing the inflammatory state – MMA (Rekiel 2003). 158 A. Rekiel et al. TABLE 3. Chemical, physical and cytological parameters of colostrum and milk of the sows Group of sows Significance lean fat Trait between the groups x x SEM SEM Colostrum Dry matter 25.86 0.646 25.81 0.475 ns Solids-not-fat 19.31 0.390 19.82 0.609 ns Fat 5.99 0.420 5.81 0.366 ns % Protein 14.07 0.232 14.14 0.288 ns Lactose 3.77 0.388 3.59 0.327 ns SCC – 1.55·106 0.630·106 1.49·106 0.479·106 ns SCC Logarithm – 5.47 0.204 5.50 0.139 ns Milk Dry matter 18.67 0.316 19.10 0.277 ns Solids-non-fat 13.04 0.662 13.27 0.580 ns Fat % 7.31 0.235 7.45 0.206 ns Protein 4.84 0.183 4.94 0.145 ns Lactose 5.49 0.202 6.05 0.133 * SCC – 0.84·106 0.389·106 0.88·106 0.34·106 ns SCC logarithm – 5.31 0.161 5.41 0.120 ns pH – 5.87 0.107 6.07 0.099 ns *Significant at P ≤0.05, ns – differences statistically insignificant. The obtained results of the studies confirm the justness of controlling the fat reserves in the reproduction managed sows, constituting the background for assessment of condition, in spite of the fact that the effect of fatness of females on the content of basic constituents of colostrum and milk was not found. In case of higher fat reserves in body of the sows, only a significant increase of lactose content in milk was recorded. of lactose in favour of group F vs group L (0.56 p.p.). The mentioned result indicates the need of continuing the studies as lactose is a valuable, easily assimilated component of mother feed for the newborns. 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TUMMARUK P., TANTASUPARUK W., TECHAKUMPHU M., KUNAVONGKRIT A. 2007: Age, body weight and backfat thickness at first observed oestrus in crossbred Landrace × Yorkshire gilts, seasonal variations an their influence on subsequence reproductive performance. Anim. Reprod. Sci. 99 (1–2): 167–181. WALKIEWICZ A., WIELBO E., KAMYK P., STASIAK A., 1994: Wpływ rozwoju somatycznego loszek na początkową rozrodczość, zdolność laktacyjną i skład chemiczny mleka. Ann. UMCS, Lublin – Polonia, EE 12, 12: 81–87. WHITTEMORE C.T., ETIENNE M., DOURMOND J.Y. 1995: Nutrition and body condition in relation to productivity. 46th Ann. Meet. EAAP, Prague, 4–7 September, P 4.5, 331. Streszczenie: Wpływ grubości słoniny w punkcie P2 przy inseminacji na wybrane parametry siary i mleka loch. Badaniami objęto 32 samice F1 (pbz × wbp), inseminowane nasieniem knurów rasy duroc. Lochy przydzielono do grupy L (chude), o średniej grubości słoniny w punkcie P2 ≤18 mm (n = 16 sztuk) lub grupy F (grube), o P2 >18 mm (n = 16 sztuk). Obserwacje prowadzono od dnia skutecznego pokrycia/inseminacji loch, przez okres ciąży i odchowu potomstwa, do odsadzenia prosiąt w 35. dniu odchowu. Kontrolowano otłuszczenie loch (pomiary grubości słoniny w punktach P2 i P4) i ich umięśnienie (wysokość mięśnia najdłuższego grzbietu w punkcie P4M) przy pokryciu oraz w ciąży wysokiej (104 ±1–2 dzień ciąży). Od wszystkich loch pobrano próby siary i mleka (łącznie 64 próby). Określono podstawowy skład chemiczny, liczbę komórek somatycznych – LKS oraz kwasowość czynną – pH. Grubość słoniny w punkcie P2 nie miała wpływu na skład chemiczny siary i mleka, LKS i pH, mimo to kontrola kondycji loch jest zasadna. Wykazano bowiem istotną różnicę (P ≤0,05) w zawartości laktozy w mleku (21. dzień) na korzyść grupy F względem grupy L (0,56 p.p.). Wynik ten wskazuje na potrzebę kontynuacji badań, gdyż laktoza jest wartościowym, łatwo przyswajalnym składnikiem pokarmu matczynego dla noworodków. Słowa kluczowe: lochy, słonina w punkcie P2, jakość siary i mleka MS received November 2015 Author’s address: Anna Rekiel Zakład Hodowli Trzody Chlewnej Katedra Szczegółowej Hodowli Zwierząt Wydział Nauk o Zwierzętach SGGW ul. Ciszewskiego 8, 02-786 Warszawa Poland e-mail: [email protected]