Report on the BioImagine “Nencki Microscopy Workshop

 Report on the BioImagine “Nencki Microscopy Workshop”
organizers: Grzegorz Wilczyński, Jakub Wlodarczyk, Tytus Bernaś
The BioImagine event “Nencki Microscopy Workshop took place at the Nencki Institute on
May 28th-31st. The workshop comprised both, lectures and training in the advanced
microscopy systems and techniques available at the Nencki Institute. The main aim of the
event was to present a frontier microscopy research and methodologies to the broad audience.
The lectures were given by international microscopy experts who covered a broad
array of techniques: Yannick Schwab from EMBL, Heidelberg (correlative microscopy),
Jonas Ries from EMBL, Heidelberg (superresolution microscopy), Edward Lemke from
EMBL, Heidelberg (novel biosensors for cell metabolism), Evgeni Ponimaskin from
Medical University of Hannover (imaging of plasma membrane receptors), Andre Zeug
from Medical University of Hannover (FRET in cell biology), Yuri Prokazov from
Leibniz Institute, Magdeburg (FLIM detectors), Andrew Woehler from Max Planck
Institute, Goettingen (multiplex FRET biosensors), and Ireneusz Grulkowski from Nicolaus
Copernicus University, Toruń (mesoscopic imaging by optical coherence tomography) (see
the program below). In addition to full length lectures, short talks were given by speakers
selected from participants, based on the quality of research presented in abstracts: Matthia
Winter-Karreman, Adriana Magalska, Alexander Wirth, Michał Stawarski, Antonina
Khoruzhenko, Kamil Parobczak, Agnieszka Walczak.
The practicals were organized in the microscopy core facility of the Nencki Institute,
and given by experienced microscopists. The following systems and techniques were
presented: 1) Live-cell confocal imaging of primary neuronal cultures, by Joanna Dzwonek,
Anna Skupień, and Marta Pyskaty, on Zeiss 780 LSM, 2) Fluorescence correlation
spectroscopy, by Jędrzej Szymański, on Leica TCS SP8, 3) Automatic 3D reconstructions in
electron microscopy, by Wojciech Brutkowski and Paweł Trzaskoma, on Zeiss Sigma
FESEM equipped in 3View system of Gatan, 4) “Clarity” technique of imaging of transparent
brain, by Michał Stawarski, using Zeiss-Yokogawa spinning disk microscope, 5) Highpressure freezing and freeze-substitution processing of tissue for electron microscopic
immunogold studies, by Andrzej Szczepankiewicz and Kamil Parobczak, using Leica
EMPACT 2 and AFS machines, 6) Fluorescence life-time imaging, by Jarosław Korczyński,
on Leica TCS SP8.
The number of partcipants attending the workshop was 103 (see the list of names at
the end of this document). The participants came from various academic centers of Poland,
from Ukraine and from Germany.
The poster:
Pictures:
Ireneusz Grulkowski “Multidimensional interferometric imaging and metrology in biomedicine – from microscopy to mesoscopy” Yannick Schwab “Correlative Light and Electron Microscopy: taking snapshots of the living at the ultrastructural level” Matthia Winter‐Karreman “Lights Will Guide You: Tracking Invasive Tumor Cells with Correlative Intravital Fluorescence Microscopy and Electron Microscopy” Evgeni Ponimaskin “Regulation of receptor functions by heterodimerization of serotonin receptors” Jonas Ries “Superresolution Microscopy of Protein Structures in Situ” Adriana Magalska “Loss of neuronal 3D chromatin organization causes transcriptional and behavioral deficits related to serotonergic dysfunction” Edward Lemke “Decoding molecular plasticity from single molecules to large assemblies” Andre Zeug “How to visualize protein’s function: Quantitative molecular microscopy approaches” Alexander Wirth “ Cellular functions of Cdc42 are dependent on lipid modifications Yury Prokazov “From large field microscopy to single photon counting” Andrew Woehler “Mulitsensor FRET microscopy: towards spatiotemporal correlation of intracellular signaling processes” Antonina Khoruzhenko “mTOR association with nucleoli and intermediate filaments in human normal and breast cancer cells” Katarzyna Rojek “Expression and function of Angiomotin family of proteins in the brain” Kamil Parobczak “Arc functional neighborhood in the nucleus” Agnieszka Walczak “Spatial repositioning of the Bdnf gene upon seizures” Błażej Ruszczycki “Before practicals: computational methods in (neuro)biological image analysis” The audience Practicals: live‐cell imaging Practicals: 3View Practicals: Clarity