Determination of Antibiotic Resistance Profile and Frequency of
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Determination of Antibiotic Resistance Profile and Frequency of
ƱŚººŬƳŻƾĪºƃżěƭƺººƬƗƵŚĮºƄƳřŵƾººƄƷƹĦěƾººưƬƗƽƶººƬŬƯ ÕÒŚţÔÔšŚŰºƠƇÎÐÖÐƱŚºŝōƹźºƸƯÖÐƽƵŹŚ ºưƃÏÏƽƵŹ ƹŵ Downloaded from zums.ac.ir at 1:33 IRST on Tuesday March 7th 2017 ŽŚƳƺƯƹŵƺſƽŚƷƶƫƹż ƿřŹŵ ŚƷŻŚƯŚŤĩLJŚŤŝƺƫŚŤƯƾƳřƹřźƟƹƾĪǀţƺǀŝƾ ŤƳōŢƯƹŚƤƯƽƺĮƫřƲǀǀƘţ řŻƺƴƿĥƹźŗō ÐƾưƜǀƋŜǀŞůźŤƧŵÐƾƤůƽźŴƟźŤƧŵÏƾƳŚŬƳŻƽŹƹźſƮǀůŹźŤĩŵÎƾŤưƷƶưƏŚƟ [email protected] ƾſŚƴƃśƹźƨǀƯƵƹźĭ řƶǀƤŝƾƨƃżěƭƺƬƗƵŚĮƄƳřŵƱřźƸţƩƹƺƀƯƽƵŶƴƀƿƺƳ ÖÏÎÏÎÎƁźƿŸěÖÏÔÔŢƟŚƿŹŵ ƵŶǀĪģ ƱƹżºƟř ŻƹŹ ŢºƯƹŚƤƯ ƶŝƶūƺţŚŝŢſřƾƳŚŤſŹŚưǀŝƽŚƷŢƳƺƠƗƮƸƯƪƯřƺƗŻř ƾĪƿƹŜƬƏŢƇźƟƾƳĥƺţŚěřŻƺƴƿĥƹźŗōŽŚƳƺƯƹŵƺſƝŶƷƹƶƴǀƯŻ ƝŶƷŵƺƃƾ ƯƱřŶƴģ ƹŵ ƽźŤĩŚŝƲƿř ŢƯƹŚƤƯ ŢǀưƷř ƭŚŤĩLJŚŤŝ šŚŞǀĩźţƶŝ ŢŞƀƳ ƅƺƈų ƶŝ ƹ ƾŝƹźĪǀƯŶƋƽŚƷƹŹřŵƶŝřŻƺƴƿĥƹźŗōŽŚƳƺƯƹŵƺſ řŶūƽřŻƺƴƿĥƹźŗōŽŚƳƺƯƹŵƺſƽŚƷƶƫƹ żƿřŹŵ IMP, SPM, SIMƽŚƷŻŚƯŚŤĩLJŚŤŝƺƫŚŤƯƾƳřƹřźƟƹƾĪǀţƺǀŝƾ ŤƳōŢƯƹŚƤƯƽƺĮƫřƲǀǀƘţƶƘƫŚƐƯƲƿřŻř ŵƺŝƾĜǀţƺƳĥƹƾĜǀţƺƴƟƁƹŹƹŵŚŝƱŚŬƳŻźƸƃƽŚƷƱŚŤſŹŚư ǀŝƾƴǀƫŚŝƽŚƷƶƳƺưƳŻřƵŶƃ ƽŚººƷƱŚŤººſŹŚưǀŝŻřƞººƬŤŴƯƾƴǀƫŚººŝƽŚººƷƶººƳƺưƳŻřřŻƺººƴƿĥƹźŗōŽŚƳƺƯƹŵƺººſƶººƫƹżƿřÎÏÍƾº ƘƐƤƯƾƠǀº ƇƺţƽƶººƘƫŚƐƯƲº ƿřŹŵƾº ſŹźŝƁƹŹ 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P, Wieczorek P, Hauschild T, Zórawski Clusters of imipenem-resistant Acinetobacter M, Olszañska D, Tryniszewska E. Metallo-beta- baumannii lactamases of Pseudomonas aeruginosa: a novel carbapenemases in an intensive care unit. Clin mechanism resistance to beta-lactam antibiotics. Microbiol Infect. 2008; 14: 588-94. Folia Histochem Cytobiol. 2008; 46: 137-42. 5- Pitout JDD, Gregson DB, Poirel L, McClure 2- Flalagas ME, producing different DE. JA, Le P, Church DL. Detection of Pseudomonas â-lactamase-producing aeruginosa producing metallo-beta-lactamases in Organisms. J Hospital Infection. 2009; 73: a large centralized labratory. J Clin Microbiol. 345-54. 2005; 43: 3129-35. 3- Wilke MS, Lovering AL, Strynadka NCJ. Â- 6- Mendiratta DK, Deotale V, Narang P. Metallo- lactam antibiotic resistance: a current structural beta-lactamase perspective. Curr Opin Microbiol. 2005; 8: aeruginosa in a hospital from a rural area. Indian 525-33. J Med Res. 2005; 121: 701-03. Extendedspectum Karageorgopoulos clones producing Pseudomonas ÎÐÖÐƱŚŝōƹźƸƯÖÐƽƵŹŚưƃ ÏÏƽƵŹ ƹŵ ƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽƶƬŬƯ řŻƺƴƿĥƹźŗōŽŚƳƺƯƹŵƺſƽŚƷƶƫƹżƿřŹŵŚƷŻŚƯŚŤĩLJŚŤŝƺƫŚŤƯƾƳřƹřźƟ 7- Gupta V. Downloaded from zums.ac.ir at 1:33 IRST on Tuesday March 7th 2017 Pseudomonas in negative bacteria from a university hospital in Acinetobacter Southern Taiwan. Int J Antimicrob Agents. 2008; Metallo-beta-lactamases aeruginosa and 84 species. Investing Drugs. 2008; 17: 131-43. 32: 475-80. 8- Clinical and Laboratory Standards Institute. 15- Varaiya A, Kulkarni N, Kulkarni M, Bhalekar Performance standards susceptibility testing; for antimicrobial P, Dogra J. Incidence of metallo-beta-lactamase 21th informational producing Pseudomonas aeruginosa in ICU supplement. CLSI document M100-S21. 2011. patients. Indian J Med Res. 2008; 127: 398-402. 9- Elizabeth BH, Vincent HT. Impact of 16- Sader HS, Reis AO, Silbert S, Gales AC. multidrug-resistant aeruginosa IMPS, VIMs and SPMs: the diversity of metallo- infection on patient outcomes. Expert Rev â-lactamases produces by carbapenem-resitant Pharmacoecon Outcomes Res. 2010; 10: 441-51. Pseudomonas aeruginosa in a Brazillian hospital. 10- Saderi H, Lotfalipour H, Owlia P, Salimi H. Clin Microbiol Infect. 2005; 11: 73-6. Detection 17- of Pseudomonas metallo-â-lactamase producing Mihani F, Khosravi A. Isolation of Pseudomonas aeruginosa isolated from burn Pseudomonas aeruginosa strains producing patients in Tehran, Iran. Science. 2010; 41: 10. metallo-beta -lactamases from infections in 11- Imani Foolad AA, Rostami Z, Shapouri R. burned patients and identification of blaIMP and Antimicrobial resistance and ESBL prevalence in blaVIM genes by PCR. Iranian J Med Microbiol. Pseudomonas aeruginosa strains isolated from 2007; 1: 23-31. clinical specimen by phenotypic and genotypic 18- Shakibaie MR, Shahcheraghi F, Hashemi A, methods, Zanjan, Iran. Iranian J Med Microbiol. Adeli NS. Detection of TEM, SHVand PER type 2011; 10: 189-98. extended-spectrum- 12- Gomes FMR, Caiaffa-Filho HH, Burattin MN, clinical strains of Pseudomonas aeruginosa Rossi among isolated from burnt patients at shafa-Hospital, imipenem-resistant Pseudomonas aeruginosa in a Kerman,Iran. Iranian J Med Sci. 2008; 11: 49-54. brazilian university Hospital. Clin Sci. 2010; 65: 19- Arunagiri K, Sekar B, Sangeetha G, John J. 825-29. Detection and characterization of metallo-â- 13- Ellington MJ, Kistler J, Livermore DM, lactamases Woodford N. Multiplex PCR for rapid detection phenotypic and molecular methods from clinical of genes encoding acquired metallo-b-lactamases. samples in a tertiary care hospital. West Indian J Antimicrob Chemother. 2007; 59: 321-22. Med J. 2012; 61: 778. F. Metallo-beta-lactamases in lactamase Pseudomonas genes among aeruginosa by 14- Lee MF, Peng CF, Hsu HJ, Chen YH. Molecular characterization of the metallo-âlactamase genes in imipenem-resistant gram- ÎÐÖÐƱŚŝōƹźƸƯÖÐƽƵŹŚưƃ ÏÏƽƵŹ ƹŵ ƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽƶƬŬƯ 85 ƱřŹŚƨưƷƹƾŤưƷƶưƏŚƟ Determination of Antibiotic Resistance Profile and Frequency ofMetalloBeta- Lactamases in Pseudomonas Aeruginosa Isolates Hemmati F1, Sourouri Zanjani R2, Haghi F1, Zeighami H1 1 Downloaded from zums.ac.ir at 1:33 IRST on Tuesday March 7th 2017 Dept. of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran 2 Dept. of Microbiology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Corresponding Author: Sourouri Zanjani R, Dept. of Microbiology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran E-mail: [email protected] Received: 29 Sep 2013 Accepted: 2 Mar 2014 Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen and one of the most frequent pathogens in nosocomial infections. This bacterium shows high resistance to majority of antibiotics including â-lactams.The aim of this study was to investigate the pattern of antimicrobial resistance and evaluation of IMP, SPM and SIM Metallo- Beta- Lactamase producing Pseudomonas aeruginosa, in clinical samples isolated from Zanjan hospitals, by phenotypic and PCR methods. Materials and Methods: In this cross-sectional study, 120 Pseudomonas aeruginosa isolates were collected from the various clinical specimens in Zanjan hospitals from 2013 to 2014. After identification of isolates using biochemical tests, the antibiotic susceptibility test (Kirby-Baur method) was done according to CLSI advice against 7 antibiotics. The Combined Disk method was then carried out for detection of MBLs and the blaIMP, blaSPM and blaSIM genes were determined by a PCR method. Results: In this study, Cefotaxime and Amikacin with 43.3% and 21.6% showed the highest and lowest resistance against isolates, respectively. From a total of 120 isolates, 35 (29.2%) strains were imipenem resistant and metallo- beta-lactamase producer. From 35 MBL producing isolates, 28 (80%) strains, 20 (57.1%) strains and 5 (14.3%) strains carried blaIMP, blaSPM and blaSIM genes, respectively and 1 (2.8%) isolate carried all blaIMP, blaSPM and blaSIM genes. Conclusion: According to the results, high prevalence of resistance to aztreonam and imipenem can be a warning against treatment process and distribution of resistance to other strains. Keywords: Antibiotic resistance, Mtallo-Beta-Lactamase, Pseudomonas aeruginosa ÎÐÖÐƱŚŝōƹźƸƯÖÐƽƵŹŚưƃ ÏÏƽƵŹ ƹŵ ƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽƶƬŬƯ