W. Nogala, M. Siek, K. Matuła, M. Podrażka, J. Paczesny, J

W. Nogala, M. Siek, K. Matuła, M. Podrażka, J. Paczesny, J

Nanopipettes in Scanning Ion Conductance Microscopy.
Surface Charge Mapping
W. Nogala, M. Siek, K. Matuła, M. Podrażka, J. Paczesny, J. Niedziółka-Jönsson
Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland
[email protected]
Introduction
Electrical double layer at charged interfaces
Glass pipettes have been used as scanning electrochemical microscopy (SECM)[1] tips under various condition. If the filling solution inside a
pipet is immiscible with the external solvent, a liquid/liquid interface is formed at its tip. The charge-transfer processes can occur across such
an interface, allowing feedback mode SECM experiments.[2] SECM with pipette tip was used for ion transfer kinetic measurements,[3] high
resolution topography imaging,[4] and reactivity imaging.[5]
Debye length
More recently, glass nanopipettes were utilized in scanning electrochemical cell microscopy,[6] and scanning ion conductance microscopy
(SICM). The last technique can be used for imaging the topography of biological samples[7] or surface charge mapping of solid substrates
immersed in diluted electrolytes.[8]
Glass nanopipettes obtained by capillary pulling
wikipedia.org
Surface charge mapping of living E. coli in PBS
Scanning ion conductance microscopy (SICM) - hopping mode
Yeast cells on polylysine film in saline
References
[1] M. V. Mirkin, W. Nogala, J. Velmurugan, Y. X. Wang, Phys Chem Chem Phys 2011, 13, 21196.
[2] Y. X. Wang, K. Kececi, J. Velmurugan, M. V. Mirkin, Chem Sci 2013, 4, 3606.
[3] P. Sun, Z. Q. Zhang, Z. Gao, Y. H. Shao, Angew Chem Int Edit 2002, 41, 3445.
[4] F. O. Laforge, J. Velmurugan, Y. X. Wang, M. V. Mirkin, Anal Chem 2009, 81, 3143.
[5] C. L. Lin, J. Rodriguez-Lopez, A. J. Bard, Anal Chem 2009, 81, 8868.
[6] N. Ebejer, A. G. Guell, S. C. S. Lai, K. McKelvey, M. E. Snowden, P. R. Unwin, Annu Rev Anal
Chem 2013, 6, 329.
[7] T. Ushiki, M. Nakajima, M. Choi, S. J. Cho, F. Iwata, Micron 2012, 43, 1390.
[8] K. McKelvey, S. L. Kinnear, D. Perry, D. Momotenko, P. R. Unwin, J Am Chem Soc 2014, 136,
Conclusions
-
Simultaneous nanoscale imaging of topography and
surface charge of living bacteria in their natural
enviroment can be done with SICM
Acknowledgements
IP2012 048872
UMO-2012/05/D/ST4/01956
-
E. coli bacteria in phosphate buffer saline are negatively
charged
NanOtechnology, Biomaterials and aLternative
Energy Source for ERA integration
FP7–REGPOT–CT–2011–285949-NOBLESSE