The SAXS studies of complexation dicationic gemini surfactants with

ISSRNS 2014: Abstractts / Synchrotronn Radiation in Natural
N
Sciencee Vol. 13, No. 1--2 (2014)
P-04
P--05
The SAXS studies of coomplexation
n dicationic
gemini surffactants with low moleccular nucleicc
acids DNA and siRNA
SAX
XS studies of
o zwitterion
nic lipoplexees
– nanosystem
n
based on ph
hospholipidss
and
d surfactants as innovattive delivery
y systems
for gene therap
py
W. Andrzejew
wska*, M. Skkupin and M. Kozak
K
P. Egierska,
E
M. Skupin*,
S
B. Urrban*, J. Wolaak,
W. Andrzejewska
A
a, Z. Pietralik and M. Kozak
k
Department off Macromolecullar Physics, Faaculty of Physiccs, A.
Mickiewicz Unniversity, ul.Um
multowska 85, 61-614
6
Poznań,
Poland
Departament of Moolecular Physiccs, Faculty of Ph
hysics, Adam
Mickkiewicz Universsity, 85b, Umulltowska, 61-614
4 Poznań,
Pola
and
Keywords: geemini surfactantts, DNA, siRNA
A, gene therapyy
*email: andrzzejewskaw@gm
mail.com
Keywords: small anngle scattering of synchrotron radiation, genee
theraapy, sulfobetainne derivatives
Specificc physico-cheemical properrties of dicatiionic
(gemini) surffactants are thhe reason for a broad theirr use
in studies ass delivery sysstems of theraapeutic moleccules
(i.e. nucleicc acids) intoo cells. To support the cell
transfection process, the carrier
c
molecuules must connnect
with a therappeutic molecuules and form stable compllexes
[1,2]. Due to the self-aassembly proocess, in soluution
complexes between
b
nucleic acids and gemini
g
surfactants
are organized in differentt structural foorms, such ass for
example: lam
mellaes, micellls or hexagonal phase.
Very prromising methhod to study of
o such system
ms is
small angle scattering of synchrotron radiation
r
(SA
AXS).
This methodd is able to demonstrate
d
s
structural
chaanges
occur within the studied deelivery system
ms [3,4].
The preesented here experimental
e
r
results
concernn the
structural analysis
a
(SR
R-SAXS) of
o
mixtures of
bis(1-dodecyyl imidazole) derivatives with
w low moleccular
DNA and RN
NA. The preseence of characcteristic diffracction
peaks (see Figure
F
1) inddicates probaable formationn of
cubic and hexxagonal phasees in the studied solutions.
*[email protected] ;*[email protected]
In
I the recent years theree has been a number off
tech
hnological breeakthroughs tthat have allo
owed clinicall
trialls in gene therapy [1].. Progress in
i molecularr
med
dicine, especiaally discoverinng new routess and methodss
for gene deliverry has proviided promisin
ng means off
treatment of geneetic disorders and cancers. Developmentt
of effective
e
genee carriers whicch can increaase specificityy
of taargeting and decrease
d
harm
mfulness to adjjacent healthyy
tissu
ues is very im
mportant. Suiitable for thiss purpose aree
thou
ught to be delivery
d
nanoosystems bassed on lipid-surffactant mixturres [2]. The aim of this study was too
deteermine the poossibility to uuse amphoteriic surfactantss
(zwitterionic alkkyl derivativees of sulfobetaine [3]) ass
com
mplexing agennts for nucleic acids, with
w
potentiall
appllications for gene
g
deliveryy [4]. A seriess of the smalll
anglle scattering of synchrotrron radiation
n (SR-SAXS))
meaasurements was
w performed on selecteed lipoplexess
baseed on short DNA
D
duplex (21 bp) and
d zwitterionicc
surffactants.
The
T poster prresents the sttructural anallysis of thesee
DNA
A/zwitterionicc surfactant systems on the basis off
scatttering data.. The SAX
XS data for
f
selectedd
DNA
A/zwitterionicc surfactant syystems were collected P122
beam
m line of EM
MBL Hamburgg Outstation on
o PETRA IIII
storrage ring at DESY. T
The measureements weree
perfformed on sollutions in 10 m
mM sodium phosphate
p
pH
H
7.5 with differentt DNA/surfacttant molar ratiio.
Ack
knowledgmentss: The study waas supported by research grant
„GE
ENERACJA PR
RZYSZŁOŚCI” from Ministry of Science andd
High
her Education (Poland) – decission: 12/POIG/G
GP/2013.
Figure 1.
Acknowledgm
ments: The reseearch was suppported by reseearch
grant (UMO--2011/01/B/ST55/00846) from National Science
Centre (Poland).
____
_______________________________________
___________
_______________________________________________________
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C. E. Samuell, C.R. Safinya,, Curr. Med. Ch
hem. 11 (2004))
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K Szpotkowskii, A. Kozak, R.
R Zieliński, D..
Wieczorek, M.
M J. Gajda, R
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