Natural inhibitors of metalloproteinases from various families of MA
Transkrypt
Natural inhibitors of metalloproteinases from various families of MA
ANNALES VOL. XXIV, N 3, 8W 2011 !"#$"%&'"(")*+-%)"/%"&' (45 )+"#)67'899::(;4"'4+ <=<>?<=<' < <=@' ? B < Natural inhibitors of metalloproteinases from various families of MA clan from wood degrading fungi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opyright © 2011 Medical University in Lublin 210 D&%D!?D&--"#D'+(%D4#D&x%)"'H+4B%D) R Materials. $ !(" ;+"% ! -+ "H !(H" - #44#+ " !%% (;4"5 $ " ! "%#"%'!>(H4(4(44#"(;4"'-H->$(%"$ +"(5 T#% - + ! !%$ !(" ;+"% + "' ' " % #%%' !D !(" ;+"%!+%4%(%$%5 Extraction of inhibitors of metalloproteinases T#"-%H("++;&$H4"%!Enterococcus!#4"%"$";""#"/"&!#$ !#"5 >(H"' ;$ !(";+"% + "' - T#+' ;& #4"' -"$ " %4/5T#"%-!+-"$$4'$4'-+956"%R4;(!!R ]595$#/+\4-%!D+"+P]Q5 $4 T#% - ##+ + ""+ ;- #$4! + -' + $ ;-$&4#+-5(!!T#%-!#"+;&H4\4"#$H$& $+T?[954""&+R4&%%'!/"%"4"+"\#"!#(+%' -4%!+P%(4%%$-Q5 Determination of metalloproteinase inhibitory activity $";"&#"/"&-%%(+-"$$+"\+$++%#";+"P69Q5?44/4%! 44"%"$";"#"/""%-+"+;&$H4"%+)"%"$";""H%% ##+"HP^Q'-"$< 4?4&p""4"++%(##"&4 4 4$p""4"+' %#"/4&5$""$";"&#&-%#$#"D+;&"44"%"#"5 $";""!6'8':'7';#"4#44H%+$4&%"-4&D+ ##+"HP66Q5 H4' $ %4 %%&_ 958[ 4 ! %4 -% "#(;+ -"$ $ % /4( ! " 44"% P6' 8' :' 7' ;#"4 #44H% $4&%"Q PY`HZ4Q +"%%4/+ " 956 H4&#"R4 ;(!! PR ]5[Q' :]f' ! :9 " P44 D&%!"H' Q5 !"#(;"'95[4!956j%4("! D+&"H+ #44H P+ $H4;" " $ #% ! $4&%"Q P;$ ! "H' Q +"%%4/+ " $ %;(!!-%++++$"T(-%"#(;+!^9"5$$#"-%%+;& ++""!84![j"#$4#"##"+54%-#"!(H+6[999H!69"'+$ ;%;#!$%(-%%(+[89P8Y9"$#%!$4&%"Q5$ D&%++%%&_958[4!%4-%4#+;&+"%"44+-5;%(!"$";"" 4/4%' $ " P"%%( "$";"% ! %Q - 4% (%+' % #%% !#5$ +%#";+;/##"%!D&%H/"#%!;%;# [89!T"4& 9599[[89Z"5$#H!"$";""-%#4#(4+%!44-%_k [89!D&%++ Pm [89!#4Q [89!%4qZk [89!D&%++qT699jP$%#4#(4" 280Q5 (4"$";"%!44"%%!/"(%!"4"%! #4!555 211 RESULTS /4 44"% "$";"% - 4""& "%4+ ! !(" ;+"% + " ! % -+ "H !(H" %#"%5 >(H(% + "( ! 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[5[ 8.0 3.8 11.3 0 65[ 0 2.9 33.2 [Y5^ 62.7 78.3 23.6 18.8 28.0 675W 68.6 785[ Y5W 7[58 885[ 29.0 8[58 28.8 31.0 [659 [75: ^^5W 18.0 17.2 19.7 18.6 19.3 11.3 6.8 9.7 3.0 8.0 [59 ]5[ 17.6 10.0 9.3 12.1 W5[ 0 W59 0 ;4:5"%!"$";"&#"/"&!44"%"$";"%!!(";+"%+" -+%44"%%!#4 6'8':'7';#"4#44H%+ $4&%"5$"$";"%4%-"#(;+-"$"44"%'+"$";"&#"/"& -%%(+'%+%#";+""4%+$+% (#! 44"% $4T#%_ Cerrena unicolor !(";+& "( Flammulina velutipes !(";+& "( Phlebia radiata !(";+& "( (!!T#%_ Cerrena unicolor !(";+& "( Flammulina velutipes !(";+& "( Phlebia radiata !(";+& "( $";"#"/"&Pj!"$";""QH"% 6 8 : 7 collagenase $4&%" 28.2 :85W 36.2 [75: 8[5] :95[ 37.2 ^65[ 23.6 18.8 30.7 33.2 ::5[ W65Y ]85[ 91.3 Y[5W 86.2 ]:5W 88.6 885[ 29.0 :[5^ :]5W 8Y5W :[59 37.0 38.2 [[59 [Y58 W95[ 38.0 18.0 17.2 28.6 27.0 WW5[ 37.9 ^W5^ 62.7 Y85W ][57 62.7 [65[ 28.0 675W ^W56 [Y59 37.3 :W59 18.2 785[ :[59 W959 675W Y[5: 8[58 28.8 [[57 [759 8[59 :85[ 6[5^ 18.2 28.3 29.0 18.2 20.0 [59 ]5[ W:5Y W]58 (4"$";"%!44"%%!/"(%!"4"%! #4!555 213 %(4% ;"+ -"$ + R' ! /"%"4 "+"\#"' %(HH% $ "%4+ %(;%#%%""4$&+#$"%+4&"##"+%!Piptoporus betulinus (in case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and in vivo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iptoporus betulinus5"%#"5"#$45"#$5^^'8]WY'89985 9. "5B545_Keumsa linteusan%(%%%"/%"!###44%$(H$$"$";""!#44(4 +$%"+7T%%"5 "444%&%%6:'66:'89975 86W D&%D!?D&--"#D'+(%D4#D&x%)"'H+4B%D) 10. 5'"=R5_&%""$";"+&%"4")%#"/"&""%(;H#H- rice ( 5Q#4"4%54#"569^'W:'677[5 11. ;+5'(4"B5'44#$B5_ %%&%!"T44"%%P%Q#"/""%_/"-5 "#$""Y]'8^['899[55 12. -4"H%55'4455' 5B5_/4("&!"4"%!"+%"$";"%5"#$5B5 :]Y']9['899W5 13. +% 5 #"' #%%"H + #"/" ! ;#"4 T#44(4 %%5 45 "#;"45':'6Y8['67Y75 6W5 H5B545_$";"&!!#%!Ganoderma lucidum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