PR_3_2012 po kor BK.vp:CorelVentura 7.0

Pharmacological Reports
Copyright © 2012
2012, 64, 635–642
by Institute of Pharmacology
ISSN 1734-1140
Polish Academy of Sciences
Influence of short-term L-arginine
supplementation on carbohydrate balance in rats
with ischemia-reperfusion syndrome
Hanna Krauss1, Pawe³ Bogdañski2, Przemys³aw Sosnowski1,
Joanna Suliburska3, Anna Jab³ecka4, Rafa³ Jastak1, Maciej Sassek5,
Pawe³ Maækowiak5, Artur Cieœlewicz4, Danuta Pupek-Musialik2
1
Department of Physiology, Poznan University of Medical Sciences, Œwiêcickiego 6, PL 60-781 Poznañ, Poland
2
Department of Internal Medicine, Metabolic Disorders and Hypertension, Poznan University of Medical Sciences,
Szamarzewskiego 84, PL 60-569 Poznañ, Poland
3
Department of Hygiene and Human Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31,
PL 60-624 Poznañ, Poland
4
Department of Clinical Pharmacology, Poznan University of Medical Sciences, D³uga 1/2, PL 61-848 Poznañ,
Poland
5
Department of Physiology and Biochemistr y, Faculty of Animal Breeding and Biology, Poznan University
of Life Sciences, Wo³yñska 35, PL 60-637 Poznañ, Poland
Correspondence: Joanna Suliburska, e-mail: [email protected]
Abstract:
Background: There are studies showing stimulative effect of arginine on insulin secretion. This mechanism is not fully explained.
The effects of the impact of arginine on carbohydrate balance under the conditions of ischemia and reperfusion remain to be determined. The aim of this study is the evaluation of the influence of short-term L-arginine supplementation on the concentration of glucose and insulin in blood and insulin binding in rat skeletal muscle under the conditions of ischemia and reperfusion.
Methods: The study was conducted on male Wistar rats with average body mass 250 ± 30 g. Animals were divided into four groups:
Group I – control, Group II – placebo, Group III – L-arginine 500 mg/kg/24 h for 5 days, Group IV – L-arginine and L-NAME
(75 µmol/rat/24 h) for 5 days. Each group was divided into subgroups depending on duration of ischemia and reperfusion. Acute
ischemia of hind limb was induced in each group by putting pneumatic tourniquet on the thigh. Blood samples and skeletal muscles
were collected from the rats. Plasma concentrations of glucose and insulin were measured. Insulin binding to insulin receptors was
determined in skeletal muscle.
Results: A clear reduction of insulin binding to receptor was found in the group of animals without ischemia and the group supplemented with L-arginine and subjected to 4-h ischemia and 30- and 120-min reperfusion. A significant increase in insulin level was
found in groups of animals with L-arginine and/or L-NAME subjected to 4-h ischemia at all times of reperfusion. Supplementation
with L-arginine and/or L-NAME decreased levels of glucose in blood serum of animals undergoing ischemia-reperfusion syndrome
compared to the control and placebo groups.
Conclusion: Under conditions of ischemia-reperfusion, short-term administration of L-arginine causes a decrease in insulin binding capacity of insulin receptors in skeletal muscle, an increase in insulin level and a decrease in the concentration of glucose in blood serum.
Key words:
L-arginine, ischemia, insulin, glucose
Pharmacological Reports, 2012, 64,
635–642
635