1. General information

Uniwersytet Śląski w Katowicach
Wydział
str. 1
Field of study and level: Chemistry, second level
Module syllabus: Module of specialization subjects A 0310-CH-S2-004
Name of the course: Physicochemical foundations of liquid chromatography
0310-CH-S2-158
1. General information
Module coordinator
Academic year
Semester
Form of study
Way of final
evaluation
Additional
information
Prof.dr hab. Teresa Kowalska
2013/2014
Winter
stationary
Written examination
2. Description of the subject and work for students
Name
Lecture: Physicochemical foundations of liquid
chromatography – in English
Instructor
Prof.dr hab.Teresa Kowalska
code
0310-CH-S2158_fs_1
group(-s)
Students of specialization V: „Physicochemical methods in chemical
analysis”
Lecture topics
1. General characteristics of chromatographic systems and the individual components
thereof in liquid chromatography.
2. Definition of the retention process.
3. Short classification of chromatographic techniques. Adsorption and partition liquid
chromatography in the planar and column variant.
4. Basic parameters of retention and resolution in planar and column
chromatography. Theoretical plates number (N) and separation number (SN). The van
Deemter equation.
5.Liquid chromatography in the isocratic and gradient mode.
6. Sami-empirical retention models in liquid chromatography. Characterization of
chromatographic activity of sorbents and chromatographic polarity of eluents.
7. Concepts of elution strength, and polarity index and selectivity parameters of
solvents after Snyder.
8. Optimization of separation selectivity in chromatography.
Ways to provide Lectures employing multimedial techniques.
course
No. teaching hrs 15
No. contact hrs
Number of
15
hours for
Uniwersytet Śląski w Katowicach
Wydział
students work
Description of
the student
work
Organization of
the course
Compulsory
readings
Complementary
readings
str. 2
Accumulation of knowledge within the framework established by the lecture topics.
Lecture
1.Z. Witkiewicz, “Podstawy chromatografii”, WNT, Warszawa, 2005
2.L.R. Snyder i J.J. Kirkland, ”Introduction to Modern Liquid Chromatography”, Wiley, New York, 1979
3.”Handbook of Thin-Layer Chromatography”, pod red. J. Shermy i B. Frieda, Dekker, New York, 1996
4.F. Geiss, ”Fundamentals of Thin-Layer Chromatography (Planar Chromatography)”, Dr Alfred Hűthig
Verlag, Heidelberg, 1987
5.K. Bielicka-Daszkiewicz, K. Milczewska, A. Voelkel, "Zastosowanie metod chromatograficznych",
WPP Poznań 2005.
6.Grob R.L., Barry E.F. "Modern Practice of Gas Chromatography (FourthEdition)", John Wiley & Sons.
7. E. deHoffmann, J. Charette, V.Stroobant “Spektrometria mas“ WNT W-wa
1.Marvin C. McMaster “GC/MS: A Practical User’s Guide”, John Wiley & Sons.
2.H. J.Hubschmann “Handbook of GC/MS” Wiley – VCH
3.W.Jennings “Analytical Gas Chromatography” Academic Press, Inc.
www address
Additional
information
Name
Conversatorium
code
0310-CH-S2158_fs_2
Instructor
Prof.dr hab.Teresa Kowalska
Group(-s)
Discussion
topics
Students of specialization V „Physicochemical methods in chemical analysis”
1. General characteristics of chromatographic systems and the individual components
thereof in liquid chromatography.
2. Definition of the retention process.
3. Short classification of chromatographic techniques. Adsorption and partition liquid
chromatography in the planar and column variant.
4. Basic parameters of retention and resolution in planar and column
chromatography. Theoretical plates number (N) and separation number (SN). The van
Deemter equation.
5.Liquid chromatography in the isocratic and gradient mode.
6. Sami-empirical retention models in liquid chromatography. Characterization of
chromatographic activity of sorbents and chromatographic polarity of eluents.
7. Concepts of elution strength, and polarity index and selectivity parameters of
solvents after Snyder.
8. Optimization of separation selectivity in chromatography.
Ways to provide Seminar
course
Number of
15
contact hours
Uniwersytet Śląski w Katowicach
Wydział
Number of
hours for
students work
Description of
the student
work
Organization of
the course
Compulsory
readings
Complementary
readings
str. 3
15
Students need to actively employ the information passed to them in the lecture
course, and also individually derived from the literature readings.
1.Z. Witkiewicz, “Podstawy chromatografii”, WNT, Warszawa, 2005
2.L.R. Snyder i J.J. Kirkland, ”Introduction to Modern Liquid Chromatography”, Wiley, New York, 1979
3.”Handbook of Thin-Layer Chromatography”, pod red. J. Shermy i B. Frieda, Dekker, New York, 1996
4.F. Geiss, ”Fundamentals of Thin-Layer Chromatography (Planar Chromatography)”, Dr Alfred Hűthig
Verlag, Heidelberg, 1987
5.K. Bielicka-Daszkiewicz, K. Milczewska, A. Voelkel, "Zastosowanie metod chromatograficznych",
WPP Poznań 2005.
6.Grob R.L., Barry E.F. "Modern Practice of Gas Chromatography (FourthEdition)", John Wiley & Sons.
7. E. deHoffmann, J. Charette, V.Stroobant “Spektrometria mas“ WNT W-wa
1.Marvin C. McMaster “GC/MS: A Practical User’s Guide”, John Wiley & Sons.
2.H. J.Hubschmann “Handbook of GC/MS” Wiley – VCH
3.W.Jennings “Analytical Gas Chromatography” Academic Press, Inc.
www address
Additional
informations
3. Description of verification methods
Name
code
Examination
0310-CH-S2-158_w_1
ID course
person
conducting
verification
group(-s)
Requirements –
course contents
Examination
Criteria
Additional
information
0310-CH-S2-158_fs_1, 0310-CH-S2-158_fs_2
Prof.dr hab.Teresa Kowalska
Students of specialization V: „Physicochemical methods of chemical analysis”
Accumulation of knowledge within the framework established by the lecture course.
Written exam based on twelve test questions
11 -12 correct answers; very good (bdb)
8-10 correct answers; good (db)
7-8 correct answers; satisfactory (dst)
Less than 7 correct answers; unsatisfactory (ndst)