docEffect of Nitrogen-Nano Doped of Titanium Dioxide in Human A
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Effect of Nitrogen-Nano Doped of Titanium Dioxide in Human A
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ÎÍÍŚţÍÍÎŢƔƬƛƶŝƶŤƀŝřƹšŹƺƇƶŝŢƗŚſÏÑŻřŶƘŝA-375ƽŚƷƩƺƬſŶƃŹƂƷŚĩ źŝƭźĭƹźĪǀƯÎÍÍŚţÍÍÎŢƔƬƛƶŝƶŤƀŝřƹšŹƺƇƶŝŢƗŚſÑÕŻřŶƘŝA-375ƩƺƬſŶƃŹƂƷŚĩŹŵN-TiO2źŧřśŵƺƃƾƯƾƫƺƬſ (SD)ŵŹřŶƳŚŤſřƽŚƐųtƪƤŤƀƯƂƴĩřƹƶſƲǀĮƳŚǀƯšŹƺƇƶŝŹřŵƺưƳŹŵƵŶƃƶƿřŹřźƿŵŚƤƯŵƺƃƾƯƾƫƺƬſźǀŨĪţƹŶƃŹƂƷŚĩŜŞſ źŤǀƫƾƬǀƯ ŶƃŚŝƾƯ ÎÐÖÓŢƄƸŞƿŵŹřƹƲƿŵŹƹźƟÎÍÕƽƵŹŚưƃ ÏÒƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ ƱĥƹźŤǀƳŚŝƵŶƃƆƫŚųŚƳ ŶǀƀĩřƽŵƭƺǀƳŚŤǀţ šřŹŷƺƳŚƳ 74 Downloaded from zums.ac.ir at 20:15 IRST on Tuesday March 7th 2017 ¦·Y [ ƺƬŝƱŚĜƿźţĬƳŹƖƟŵƱƺƯŻōŻřƵŵŚƠŤſřŚŝA-375ƽŚƷƩƺƬſƾƿŚŤƀƿŻźŝƾŗźƯŹƺƳźǀŧŚţŢŰţ N-TiO2źŧřƾſŹźŝÐƪĪƃ źƿŵŚƤƯŢƗŚſéíŻřŶƘŝA-375ƽŚƷƩƺƬſƾƿŚŤƀ ƿŻŹŵN-TiO2źŧřśŢƗŚſçéŻřŶƘŝA-375ƽŚƷƩƺƬſƾƿŚŤƀƿŻŹŵ N-TiO2źŧřƞƫř ŶƃŚŝƾƯ (SD)ŵŹřŶƳŚŤſřƽŚƐųtƪƤŤƀƯƂƴĩřƹƶſƲǀĮƳŚǀƯšŹƺƇƶŝŹřŵƺưƳŹŵƵŶƃƶŗřŹř ƵŶºƿŵƾŬƳŹŚºƳƲǀţŚºƯƹźĩŚºŝƾƿŚºƷƩƺƬºſƪĪƃƶŝŢƗŚſÏÑ ƹŢſřŻƺŤěƺěōƕƺƳŻřƾƫƺƬſīźƯįƵŶƴƷŵƱŚƄƳƶĩŶƳƺƃƾƯ ƞƫřêƪĪƃŶƳƺƃƾƯƵŶƿŵżŞſĬƳŹƶŝƩźŤƴĩƽŚƷ ƩƺƬſ ŻřƵŵŚƠŤºſřŚºŝŵƺºƃƾºƯƵŶƷŚƄºƯÒƪĪºƃŹŵƶºĩŹƺºƏƱŚưƷ ƵŶƃŹŚưǀţƽŚƷƩƺƬſAo/EtĬƳŹƹŢƴſŹƺƬƟęƺĪſƹźĪǀƯ ŻřžºěźºŤǀƫƾºƬǀƯźºŝƭźĭƹźĪǀƯÎÍÍŢƔƬƛŹŵN-TiO2Śŝ ÎÐÖÓŢƄƸŞƿŵŹřƹƲƿŵŹƹźƟÎÍÕƽƵŹŚưƃ ÏÒƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ ƱřŹŚƨưƷƹƾƠƿźƃƮƿźƯ Downloaded from zums.ac.ir at 20:15 IRST on Tuesday March 7th 2017 75 ŢƔƬƛƶŝƶŤƀŝřƹšŹƺƇƶŝšřźǀǀƜţMTTƱƺƯŻōŻřƵŵŚƠŤſřŚŝŢƗŚſÑÕŻřŶƘŝA-375ƽŚƷƩƺƬſźŝ N-TiO2ƾưſšřźŧřƾſŹźŝéƪĪƃ ŶƃŚŝƾƯ (SD)ŵŹřŶƳŚŤſřƽŚƐųtƪƤŤƀƯƂƴĩřƹƹŵƲǀĮƳŚǀƯšŹƺƇƶŝŹřŵƺưƳŹŵƵŶƃƶ ŗřŹřźƿŵŚƤƯŢſřźŤǀƫƾƬǀƯźŝƭźĭƹźĪǀƯ ÎÍÍŚţÎ ŹŵƱōƕƺººƣƹƭŶººƗƹŚººƷƩƺƬººſƲ ººƿřŹŵŻƺººŤěƺěōƕƺººƣƹ śÒƪĪƃƮƿŵƺŝƩźŤƴĩƽŚƷƩƺƬſ ƶººƘƐƣƶººƘƐƣƱƺººƯŻōŻřƪººƇŚůŪƿŚººŤƳŽŚººſřźººŝƲǀº ƴĤưƷ ŚºººŝŹŚºººưǀţŢºººŰţA-375ƽŚºººƷƩƺƬºººſŹŵDNAƱŶºººƃ ŶƷŚººƃźººŤǀƫƾ ººƬǀƯźººŝƭźººĭƹźĪǀƯÎÍÍŢººƔƬƛŹŵN-TiO2 [ ƽżǀƯōĬƳŹ ƹŢƴſŹƺƬƟęƺĪſƹźĪǀƯŻřƵŵŚƠŤſřŚŝA-375ƽŚƷƩƺƬſźŝƾŗźƯŹƺƳźǀŧŚţŢŰţ N-TiO2ĨǀţƺŤěƺěōźŧřƾſŹźŝƞƫřêƪĪƃ ŻřžěDNAƱŶƃƶƘƐƣƶƘƐƣƱƺƯŻōŻřƵŵŚƠŤſřŚŝA-375ƽŚƷƩƺƬſźŝƾŗźƯŹƺƳźǀŧŚţŢŰţ N-TiO2ĨǀţƺŤěƺěōźŧřƾſŹźŝśAo/Et ŻřƾĩŚůƶĩŶƃƵŶƷŚƄƯ(Smear)ƶĪƫŢƫŚůźŤǀƫƾƬǀƯźŝƭźĭƹźĪǀƯ æååŢƔƬƛŹŵN-TiO2ŚŝŹŚưǀţŢŰţA-375ƽŚƷƩƺƬſŹŵŢƗŚſ çé ŶƃŚŝƾƯƩźŤƴĩƽŚƷ ƩƺƬſŹŵƱōƕƺƣƹƭŶƗƹŚ ƷƩƺƬſƲƿřŹŵŻƺŤěƺěōƕƺƣƹ ÎÐÖÓŢƄƸŞƿŵŹřƹƲƿŵŹƹźƟÎÍÕƽƵŹŚưƃ ÏÒƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ ƱĥƹźŤǀƳŚŝƵŶƃƆƫŚųŚƳ ŶǀƀĩřƽŵƭƺǀƳŚŤǀţ šřŹŷƺƳŚƳ Downloaded from zums.ac.ir at 20:15 IRST on Tuesday March 7th 2017 ƂƠƴŝřźººƟįƶƘººƃřŹƺƌººůŹŵTiO2ŶººƃƵŵřŵƱŚƄººƳŢººƟźĭ 76 ƶŤƀºŝřƹšŹƺºƇƶŝŹLJƺƯƹźĪǀƯ éêŚţêƽŚƷŢƔƬƛŹŵUV ŦŰŝ ƭŚƳƮǀųŶŝƭƺƳLjƯƾŤǀſƺƳLjƯŚƄƴƯŚŝŢſƺěƮǀųŶŝŹƺƯƺţ ƽƹŹźºŝƱřŹŚºĪưƷƹƽƺſƺƯƎſƺţƶĩƽřƶƘƫŚƐƯŹŵƲǀƴĤưƷ ÏÕŶºƃŚŝƾ ºƯƾŤºſƺěƽŚºƷƱŚƏźºſźĮƿŵŻřźŤƄǀŝƱōƱŵřŵ źºŝƭźºĭƹźĪǀƯÎÍŚºţÍÍÎƽŚºƷŢƔƬƛŹŵN-TiO2ƶĩŶƃ ƶºŝƶūƺţŚŝêƹëŢſřƶŤƟźĭŹřźƣƶūƺţŵŹƺƯŹŚǀƀŝŚƯƺƳLjƯ Źŵçêŵƺºƃƾ ºƯŚºƷƩƺƬºſƲºƿřƽźƷŚºƓƪĪºƃŹŵźǀǀƜţƹ Śºţ ƮƿŶºƃƱōźºŝŚºƯƾºěřźţĨºǀƯŚƴƿŵƺţƺƟĨºǀƴĪţŹŵ ƽŹƺƳ ƲºƿřƽźƷŚºƓƪĪºƃŹŵźºǀǀƜţŚºŝƵřźưƷźŤǀƫƾ ƬǀƯźŝƭźĭƹźĪǀƯ ŹƺºƔƴƯƶºŝźºƋŚů įƶºƘƫŚƐƯƮǀºƴĩ ƾºſŹźŝřŹ ƾƳřŶǀƀĩřƾ ŤƳō ƱŚƄºƳDNAƱŶºƃƶºƘƐƣƶƘƐƣƱƺƯŻōƲǀƴĤưƷƹŢƴſŹƺƬƟ įƵŵŹƽƹŹźºŝƾºŗźƯŹƺºƳźǀŧŚºţŢºŰţƽŹƺºƳźĮſŚƀůĨƿ ƶºĩƽřƶºƘƫŚƐƯŹŵŵźºǀĭƾ ƯšŹƺƇŻƺŤěƺěōįŚƤƫřƽƶƐſřƹ ƶŝ ƾěřźţĨǀƯŚƴƿŵƺţƺƟĨǀƴĪţƶƘƫŚƐƯƲƿřŹŵƵŵŚƠŤſřŵŹƺƯƾƳŚƯŹŵ ƽŚƯƺƴǀºſŹŚĩHelaƾƫƺƬſƽŚƷƵŵŹƽƹŹźŝTiO2ƹTiO2 ƾŝŚºŴŤƳřƾƫƺƬſįƵŵŹŢƬƗƲǀưƷƶŝŶƃŚŝƾ ƯƺĮŴſŚěŢſƺě Źřźºƣ ƾŗźƯŹƺƳƉźƘƯŹŵŚƷƩƺƬſƶĪƴƿřŻřŶƘŝžĪƳŹŚƟƹŻŚƳ 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ŻƺºŤěƺěōŚºƷƩƺƬſƱŶƃƾƳŚƏźſŻřŹřźƟƽŚƷƵřŹŻř ƾĪƿæé ƱŶºƃ ƾƳŚƏźſƶŝźŬƴƯŶƴƿōźƟƲƿřŹŵƩLjŤųřƶƳƺĭźƷƹŢſř A-ƽŚƷƩƺƬſŹŵŻƺŤěƺěōįŚƤƫřŹƺƔƴƯƲƿŶŝŵŵźĭƾ ƯŚƷƩƺƬſ įƶƐººſřƹƶººŝƾºŗźƯŹƺººƳŹƺƌººůŹŵN-TiO2Ǝººſƺţ375 ŽźŤºſřįƶƐºſřƹƶºŝŻƺŤěƺěōįŚƤƫřŶƷŵƾ ƯűŹROSŶǀƫƺţ ƶºĩŶºƷŵƾ ƯƽƹŹƾƳŚƯŻƺǀţřŶǀƀĩřŽźŤſřŶƃŚŝƾ ƯƺǀţřŶǀƀĩř ƽŚººƷƕŚººƟŵƾƄººŴŝŢººƓŚƠůŢº ǀƟźƓŻřƂǀº ŝROSŶº ǀƫƺţ ŵźŝŹŚºĩƶºŤƟźĭšŹƺƇ įƶƘƫŚƐƯŹŵèæŶƃŚŝ ƾƳřŶǀƀĩřƾ ŤƳō ƶºŬǀŤƳŹŵƹƺǀţřŶǀƀºĩřŽźŤºſřƂƿřżºƟřŹƺºƔƴƯƶŝ N-TiO2 ƱŵźºŝƲǀºŝŻřŹŵŶºƿŶūŹŚºĪƷřŹƱřƺºƴƗƶŝ ƾƫƺƬſīźƯ ƽŚƤƫř šřŹŷƺƳŚºƳŶºƃƱŚºǀŝƶºĩŹƺºƏƱŚºưƷŶƃŚŝƾ ƯŭźƐƯƱŚƏźſ ƶĩŚŬƳōŻřŶƳƺƃƾ ƯƶŤŴǀĮƳřźŝUVįƶƘƃřźǀŧŚţŢŰţTiO2 ƾŞǀĩźţŻŚºŝƱŚºĪƯřƹŢºſřźƌºƯƱŶºŝƽřźºŝũƺºƯƩƺºƏƲƿř ƱĥƹźŤǀƳŚŝTiO2ƽŻŚſƆƫŚųŚƳŵŹřŵŵƺūƹƵźƠůƱƹźŤĪƫř ƹƾĪǀŤǀƫŚºººţŚĩƺţƺƟƅřƺºººųŭLjºººƇřƽŚºººƷƵřŹŻř ƾºººĪƿ References 4- Retsas S. 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Development stress: TiO2 of water‐soluble nanoparticles Biomarkers and for novel ÎÐÖÓŢƄƸŞƿŵŹřƹƲƿŵŹƹźƟÎÍÕƽƵŹŚưƃ ÏÒƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ ƱĥƹźŤǀƳŚŝƵŶƃƆƫŚųŚƳ ŶǀƀĩřƽŵƭƺǀƳŚŤǀţ šřŹŷƺƳŚƳ 80 Effect of Nitrogen-Nano Doped of Titanium Dioxide in Human A-375Melanoma Cancer Cell Line Sharifi M1, Moosavi MA2, Naji T1 1 Downloaded from zums.ac.ir at 20:15 IRST on Tuesday March 7th 2017 Dept. of Cell & Molecular Biology, Islamic Azad University Pharmaceutical Sciences Branch, Tehran, Iran. 2 Division of Medical Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran. Corresponding Author: Moosavi, Dept. of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetics Engineering and Biotechnology, Tehran, Iran. E-mail: [email protected] Received: 9 Jan 2016 Accepted: 6 Jun 2016 Backgrounds and Objective: Titanium dioxide (TiO2) is a potent photosensitizer in photodynamic therapy (PDT) and is activated upon ultraviolet (UV) irradiation.The aim of this study was the assessment of the effect of nitrogen –nano doped of titanium dioxide in human A-375 melanoma cancer cell line. Materials and Methods: N-TiO2 nanoparticles were prepared by mechanical mixing of urea with TiO2 powder in a 4:1 weight ratio. A-375 cells were treated with different concentrations of N-TiO2 for various time intervals in the presence or absence of visible light. The growth and viability of A-375 cells were determined by means of trypan blue exclusion test and MTT assay, respectively. Acridine orange/Ethidium bromide double staining and DNA gel electrophoresis were applied to determine apoptosis. Results: The results showed that visible light activated N-TiO2 could induce growth inhibition in a dose (0.01-100 ìg/ml) and time dependent manner. Decrease in viability was observed in 100 ìg/ml concentration and the results of fluorescent microscopy showed DNA fragmentation due to apoptotic cell death at this concentration. Conclusion: Based on the anti-cancer effects of N-TiO2 on A-375 cells, further evaluation of these nanoparticles as a new photosensitizer in photocatalytic therapy of skin cancer can be proposed. Keywords: Melanoma, Nitrogen doping, Photodynamic therapy, Titanium dioxide ÎÐÖÓŢƄƸŞƿŵŹřƹƲƿŵŹƹźƟÎÍÕƽƵŹŚưƃ ÏÒƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ
