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References
6- Casson RJ, Chidlow G, Ebneter A, Wood JP,
1- Er E, Oliver L, Carton PF, Juin PH, Manon S,
Crowston
Vallette FM. Mitochondria as the target of the
neuroprotection research in glaucoma: a review of
pro-apoptotic protein Bax. Biochim Biophys Acta
definitions
2006; 1757: 1301-11.
Ophthalmol 2012; 40: 350-7.
2- Chan DD, VanDyke WS, Bahls M, et al.
7-
Mechanostasis in apoptosis and medicine. Prog
neuroplasticity- a holistic approach and future
Biophys Mol Biol 2011; 106: 517-24.
perspectives. Neurol SCi. 2007; 257: 38-43.
3- Lossi L, Merighi A. In vivo cellular and
8- Kinugasa T, Ozaki S, Hamanaka S, Kudo N.
molecular mechanisms of neuronal apoptosis in
The effects of sciatic nerve axotomy on spinal
the mammalian CNS. Prog Neurobiol 2003; 69:
motoneurons in neonatal bax-deficient mice.
287-312.
Neurosc Res. 2002; 44: 439-46.
4- Lossi L, Cantile C, Tamagno I, Merighi A.
9- Bahadori MH, Al-Tiraihi T, Valojerdi MR.
Apoptosis in the mammalian CNS: Lessons from
sciatic
animal models. Vet J 2005; 170: 52-66.
induces apoptotic neuronal death in l5 dorsal
5- Rich T, Watson CJ, Wyllie A. Apoptosis: the
root
germs of death. Nat Cell Biol 1999; 1: 69-71.
125-30.
J,
and
Muresanu
nerve
ganglion.
Goldberg
I.
principles.
DF.
Clin
Experiment
Neuroprotection
transection
J
Translational
Neuro
in
neonatal
Cytol.
2001;
and
rats
30:
ÎÐÖÑƽŵƹŹŷōÎÍÍƽƵŹŚưƃ
ÏÐƽƵŹ
ƹŵƱ
ŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽƶƬŬƯ
Downloaded from zums.ac.ir at 15:45 IRST on Saturday March 4th 2017
ĨƿŹƹřŶǀſřƾŞƈƗŢƓŚƠŰƯźŧř
84
10- Tariot PN, Farlow MR, Grossberg GT,
against secondary damage after spinal cord injury.
Graham SM, McDonald S, Gergel I. Memantine
PNAS. 2005; 102: 3483-8.
treatment in patients with moderate to severe
18- Maghbooli M, Farhadi S, Esmaeilzadeh A,
Alzheimer disease already receiving donepezil: a
Mazloumzadeh S. Assessment of serum uric acid
randomized controlled trial. JAMA. 2004; 291:
and vitamin c levels in acute atherothrombotic
317-24.
stroke. J Zanjan Univ Med Sci. 2012; 20: 64-74.
11- Squadrito GL, Cueto R, Splenser AE, et al.
19- Hobbenaghi R, Javanbakht J, Hosseini E,
Reaction of Uric Acid with Peroxynitrite and
et al. Neuropathological and neuroprotection
Implications
of
features of vitamin B12 on the dorsal spinal
Neuroprotection by Uric Acid. Arch Biochem
ganglion of rats after the exprimental crush of
Biophys. 2000; 376: 333-7.
sciatic nerve. Diagnostic Pathol. 2013; 8: 123-30.
12- Tehranipour M, Baharara J, Mostafaee M. The
20- Deshad A, Parvizi M. The neuroprotective
neuroprotective effect of CSF intraperitoneal
effect of nepeta mentoides on axotomized dorsal
injection on alpha motor degeneration after sciatic
root ganglion sensory neuron in neonate rats. J
nerve compression in rat. Arak Uni Med Sci J.
BasiC CliniCal Pathophysiol. 2014, 2: 13-20.
2008; 12: 101-108.
21- Delshad A, Naseri M, Parvizi M, Fattah N,
13- Aoyama K, Matsumura N, Watabe M, Wang
Sharayeli M. The iranian traditional herbal
F, Kikuchi-utsumy K, Nakaki T. Caffeine and uric
medicine ostokhodus can prevent axotomy-
acid
induced apoptosis in spinal motoneurons in
for
mediate
the
Mechanism
glutathione
neuroprotection.
Neuroscience. 2011; 181: 206-15.
neonate rats. J Med Plant Res. 2011; 5:
14- Delshad A, Al-Tiraihi T. Ultrastructure of
4446-51.
apoptotic oligodendrocytes in the spinal cord of
22- Ames BN, Cathcart R, Schwiers E, Hochstein
adult rat with long-standing axotomized sciatc
P. Uric acid provides an antioxidant defense in
nerve. Folia neuropathol. 2001; 39: 125-8.
humans against oxidant- and radical caused aging
15- Koliatsos VE, Price DL. Axotomy as an
and cancer: a hypothesis. Proc Natl Acad Sci
experimental model of neuronal injury and cell
USA. 1981; 78: 6858-62.
death. Brain Pathol. 1996; 6: 447-65.
23- Drulovic J, Dujmovic I, Stojsavljev N,
16- Annanmaki T, Pohja M, Parviainen T,
Mesaros S, Andjelkovic S. Uric acid levels in sera
Hakkinen P, Murros K. Uric acid and cognition in
from patients with multiple sclerosis. Neurol.
parkinsone
2001; 248: 121-6.
disease:
A
follow-up
study.
Parkinsonism Related Disorders. 2011; 17: 333-7.
24- Patel VP, Chu CT. Nuclear transport,
17- Scott GS, Cuzzocrea S, Genovese T,
oxidative stress, and neurodegeneration. Int J Clin
Koprowski H, Hooper DC. Uric acid protects
Exp Pathol. 2011; 4: 215-29.
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ƶƬŬƯ
85
ƱřŹŚƨưƷƹŵŚƄƫŵƵŵřŻżƿżƗŚƋźǀƬƗźŤƧŵ
The Neuroprotective Effect of Uric Acid on Apoptosis Prevention of Dorsal Root Ganglion
Neurons
Delshad AA1, Heshmati M1, Hajizadeh Z1
Dept of Anatomical Sciences,Faculty of Medicine, Shahed University, Tehran, Iran
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1
Corresponding Author: Delshad AA, Dept of Anatomical Sciences, Shahed University, Tehran, Iran
E-mail: [email protected]
Received: 28 Oct 2014
Accepted: 10 May 2015
Background and Objective: The neuroprotective effect of uric acid as a natural antioxidant on
neurodegenerative diseases has been proposed repeatedly, but its antiapoptotic effect on spinal neurons has
not been examined yet. Due to the critical role of sensory neurons in the improvement of functional outcome
in neuroprotective strategies, the antiapoptotic effect of uric acid on dorsal root ganglions was investigated.
Materials and Methods: In this study, 10 two-day-old neonate rats were subdivided into experimental and
control groups. In both groups right sciatic nerve was transected. In the experimental group immediately
following surgery 10mg/kg uric acid and in control group the same volume of normal saline was injected
intraperitoneally. Five days following axotomy, bilateral L5 dorsal root ganglia in both groups were prepared
for morphometric cell counting and TUNEL assay.
Results: In the control group, in axotomized side compared to intact side, 37.41% of the sensory neurons
were lost, indicating the efficiency of axotomy model in the induction of cell death in sensory neurons. The
results of TUNEL assay approved the apoptotic type of neural cell death. Comparison of the control and
experimental groups indicated that intraperitoneal injection of uric acid could not inhibit apoptosis of dorsal
root ganglion sensory neurons.
Conclusion: This study illustrated that uric acid has not any significant neuroprotective effect on axotomized
sensory neurons of dorsal root ganglia.
Key words: Uric acid, Dorsal root ganglion, Axotomy, Apoptosis, Neuroprotection
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