The Protective Role of Estrogen and Progesterone on Gastric Tissue

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ÏÐƽƵŹ
ƶƬŬƯ
̂˼
The Protective Role of Estrogen and Progesterone on Gastric Tissue Following Gastric
Ischemia - Reperfusion in Male Rats
Keshavarzi Z1, Mohebbati R2, Alikhani V2, Bibak B1, Hadjzadeh MAR2
1
Dept. of Physiology, North Khorasan University of Medical Sciences, Bojnurd, Iran
2
Dept. of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran
Corresponding Author: Keshavarzi Z, Dept. of Physiology, North Khorasan University of Medical Sciences,
Bojnurd, Iran
E-Mail: [email protected]
Received: 8 Feb 2014
Accepted: 30 Aug 2014
Background and Objective: Ischemia-reperfusion (I/R) injury repeatedly occurs in situations such as
surgery, hemorrhage, burns, trauma, and infection. It has also been found that females were more resistant
than males, indicating that female sex hormones have a protective function. The aim of this study was to
determine the role of estrogen, progesterone, and their combination in protecting the gastric damage induced
by I/R.
Materials and Methods: 40 Wistar rats were randomly divided into four groups: intact, ischemia reperfusion (IR), IR + estradiol (1mg/kg), IR + progesterone (16 mg / kg) (P) and IR + combination of
estradiol (1mg / kg) and progesterone (16 mg / kg). After animal anesthesia and laparotomy, celiac artery
was occluded for 30 minutes and then circulation was established for 24 hours. Results expressed as mean ±
SEM and P <0.05 was considered statistically significant.
Results: The gastric acid concentration significantly increased after induction of gastric ischemia reperfusion (p <0.001). Treatment with estradiol, progesterone (p <0.05) and combined estradiol and
progesterone (p <0.01) significantly reduced gastric acid levels. The histopathological evaluation of gastric
tissue indicated the presence of gastric ulcer with active bleeding, erosion, submucosal edema and identified
inflammation in ischemia - reperfusion group. In groups treated with estrogen and progesterone, the ulcer
was not found; however, erosion, edema and inflammation of serous surfaces were observed.
Conclusion: Regarding acid-lowering and protective effects of estrogen and progesterone upon gastric tissue
following gastric ischemia-reperfusion, female sex steroids can be used to reduce gastrointestinal ischemic
disorders.
Keywords: Estrogen, Progesterone, Gastric acid, Rat, Ischemia- reperfusion
ÏÐƽƵŹ
ƶƬŬƯ

The Protective Role of Estrogen and Progesterone on Gastric Tissue

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