docĭŵƺƫō ƾŹ ƿżŤĪƿŚƾƿ LjƳƺţŹŚŝ ƹ ƾƿ Źŵ ƱŚƀƳř Ĩĩ
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ĭŵƺƫō ƾŹ ƿżŤĪƿŚƾƿ LjƳƺţŹŚŝ ƹ ƾƿ Źŵ ƱŚƀƳř Ĩĩ
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Silva M, Amaro F, Bacellar F, Raoult D. 37- Chomel BB, Boulouis H-J, Maruyama S, Molecular detection of Rickettsia felis, Rickettsia Breitschwerdt EB. Bartonella spp. in pets and typhi and two genotypes closely related to effect on human health. Emerg Infect Dis. 2006; Bartonella elizabethae. Am J Trop Med Hyg. 12: 389-394. 2006; 75: 727-31 ÎÐÖÑƽŵƹŹŷōÎÍÍƽƵŹŚưƃ ÏÐƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳ řŵƾƄƷƹĦěƾưƬƗƽƶƬŬƯ 115 ƱřŹŚƨưƷƹƵŵřŻźǀƯƶŞǀŞů Bartonella and Rickettsial Infections in Human FleaPulex Irritans) Mirzadeh H1, Ghavami MB1, Zeyghami H2, Taghiloo B3, Pour Rastgoo F1 1 Dept .of Medical Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. 2 Dept. of Medical Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. Corresponding Author: Ghavami MB, Dept .of Medical Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. E-mail: [email protected] Received: 7 Mar 2015 Accepted: 25 Jun 2015 Background and Objective: Human flea (Pulex irritans) is one of the most important human ectoparasites that transmits various pathogens and causes skin irritation by biting humans and animals. Planning control measures against this vector in various regions requires the knowledge of the pathogens within the vector. Thus, this study was carried out to identify Bartonella and Rickettsia infection of P. irritans in Zanjan province. Materials and Methods: Flea samples were collected via human baits from animal farms in Khodabande and Mahneshan districts from September 2013 to October 2014. DNA was extracted from human fleas, and a part of gltA gene for identification of Rickettsia, and ITS region for identification of Bartonella were amplified by specific primers. A number of PCR products were sequenced after purification. Results: Out of 1136 collected flea samples 1079 (94.98%) were P. irritas, 36 (3.16%) C. canis and 21 (1.84%) were C. felis. 4.9 % of human flea samples had Reckettsia infection, while no Bartonella infection was observed. Conclusion: High frequency and rickettsial infection of P. irritans in the studied areas need more attention of administrators and taking into account control measures against this vector. Although Bartonella infection was not observed in our samples, more comprehensive studies are required to verify this viewpoint. Key words: Pulex irritans, Rickettsia,Bartonella, gltA gene ÎÐÖÑƽŵƹŹŷōÎÍÍƽƵŹŚưƃ ÏÐƽƵŹ ƹŵƱŚºŬƳŻƾƳŚƯŹŵƾŤƃřŶƸŝšŚƯŶųƹƾĪƃżěƭƺƬƗƵŚĮƄƳřŵƾƄƷƹĦěƾưƬƗƽ ƶƬŬƯ
